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We narrowed to 1,383 results for: piggyBac

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Showing: 301 - 350 of 1383 results

  4. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available Since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  7. PB-U6-CNCB

    Plasmid
    #236763
    Purpose
    A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter, CAG promoter
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236763-plasmid-map-sequence-id-473487

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  8. PB-2G-CNCB

    Plasmid
    #236764
    Purpose
    A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter, human U6 promoter, CAG promoter
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236764-plasmid-map-sequence-id-473439

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  29. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172473-plasmid-map-sequence-id-342817

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  31. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187945-plasmid-map-sequence-id-373705

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  35. PB-TAC-OKMS-Cas9

    Plasmid
    #120353
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120353-plasmid-map-sequence-id-236054

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Showing: 301 - 350 of 1383 results