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We narrowed to 1,100 results for: cas12a

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Showing: 121 - 140 of 1100 results

  3. pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

    Plasmid
    #140221
    Purpose
    Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140221-plasmid-map-sequence-id-291991

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  5. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid
    #104563
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1 (D832A)
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    104563-plasmid-map-sequence-id-260085

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  6. AA008

    Plasmid
    #216003
    Purpose
    Fragmid fragment: (Cas protein) preferred over wildtype Cas12a
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas12a_v1.1 [EnAs]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216003-plasmid-map-sequence-id-431501

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  8. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Promoter
    RRM2 endogenous promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140228-plasmid-map-sequence-id-291990

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  9. WN10151

    Plasmid
    #80441
    Purpose
    Expresses human codon-optimized dead (832A) LbCpf1
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead (D832A) LbCpf1
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Mutation
    D832A
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80441-plasmid-map-sequence-id-151446

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  14. pSLQ10333

    Plasmid
    #183955
    Purpose
    CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclease-dead Lb Cas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183955-plasmid-map-sequence-id-363077

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  15. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114070-plasmid-map-sequence-id-220511

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  16. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140222
    Purpose
    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140222-plasmid-map-sequence-id-296741

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  17. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid
    #140227
    Purpose
    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    HRE (Hypoxia inducible)
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140227-plasmid-map-sequence-id-291996

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Showing: 121 - 140 of 1100 results