We narrowed to 15,037 results for: pET

1. pET28-GFP-mHP1alpha

   Plasmid

   #181912

   Purpose

   Bacterial protein expression vector for GFP-tagged HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-PLS-KP6_rpoA-U

   Plasmid

   #190967

   Purpose

   Test the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-51b(+) ChemoG5

   Plasmid

   #193805

   Purpose

   Protein production of ChemoG5 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG5
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP:A206K, T225R; HT7: E143R, E147R, L271E

   Promoter

   T7

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaM-1

   Plasmid

   #172767

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-1
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET3d::strep-AID

   Plasmid

   #15924

   Depositor

   Nina Papavasiliou

   Article

   Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

   Insert

   AID (AICDA Human)
   

   Use

   Tags

   strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-BE3∆UGI

   Plasmid

   #96943

   Purpose

   The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

   Depositor

   Daesik Kim

   Article

   Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

   Insert

   BE3∆UGI
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21d-colEwt2/Imwt2

   Plasmid

   #107127

   Purpose

   plasmid for expression of WT colicin endonuclease and immunity pair 2

   Depositor

   Sarel Fleishman

   Article

   Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

   Insert

   colEwt2/Imwt2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET3C-ARL16(CO)-His6

   Plasmid

   #216485

   Purpose

   Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

   Depositor

   Richard Kahn

   Insert

   ADP-ribosylation factor-like 16 (ARL16 Human)
   

   Use

   Tags

   includes GSGSGSHHHHHH (His6) tag at C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11b-SpyCatcher-M2e

    Plasmid

    #165990

    Purpose

    SpyCatcher-fused influenza M2 ectodomain with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-M2e
    

    Use

    Tags

    HisTag and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a His6Flag2AUF1(p37)

    Plasmid

    #135939

    Purpose

    Expresses AUF1p37 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-29

    Plasmid

    #172753

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-29
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21TtTERT(195-516)

    Plasmid

    #28172

    Depositor

    Kathleen Collins

    Article

    O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

    Insert

    Tetrahymena TERT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pET-22b(+)-UTPase

    Plasmid

    #12667

    Depositor

    SeWon Suh

    Article

    Han et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    dUTP pyrophosphatase (DUT1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET21a-mAIM2-His6

    Plasmid

    #51536

    Purpose

    Express His6-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3991 pET ubcH9

    Plasmid

    #8651

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH9 (UBE2I Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pET28a His6Flag2AUF1(p45)

    Plasmid

    #135942

    Purpose

    Expresses AUF1p45 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-C-terminal YPet

    Plasmid

    #128027

    Purpose

    Mammalian expression vector with C terminal YPet tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET48 AtGUN1-SMR1

    Plasmid

    #136361

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET48 AtGUN1-SMR2

    Plasmid

    #136362

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET48 AtGUN1-PPR1

    Plasmid

    #136359

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET48 AtGUN1-PPR2

    Plasmid

    #136360

    Purpose

    Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

    Depositor

    Tatsuru Masuda

    Article

    Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

    Insert

    cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
    

    Use

    Tags

    TRX-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET41-GST-KaiC

    Plasmid

    #68051

    Purpose

    Strain: AM5117, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiC
    

    Use

    Tags

    6xHis, GST, and S-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET15b-eGO-Matryoshka-LE-LP

    Plasmid

    #100017

    Purpose

    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpEGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET11a-p65(19-325)

    Plasmid

    #44744

    Depositor

    Gourisankar Ghosh

    Insert

    p65 (Rela Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AA 19-325

    Promoter

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pET28a-MBP-(CBX1)3-WT

    Plasmid

    #180212

    Purpose

    Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1 x3 (CBX1 Human)
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge