We narrowed to 12,366 results for: NUC

1. pENTR4 TNRC6B

   Plasmid

   #20022

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Trinucleotide repeat containing 6B (TNRC6B Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tmem214 pmRFP-N2 (1348)

   Plasmid

   #62047

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem214 (TMEM214 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDN42

   Plasmid

   #61345

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   GS linker-AsLov2-biNLS10 and PKIt NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3-FLAG-human NPM1-C1

   Plasmid

   #160693

   Purpose

   Mammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).

   Depositor

   Charles Sherr

   Article

   Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

   Insert

   NPM1 (NPM1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks NBD domain

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tmem214 pEGFP-N2 (1072)

   Plasmid

   #62026

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem214 (TMEM214 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

   Plasmid

   #170467

   Purpose

   The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Bakhache et al bioRxiv 2021

   Insert

   Nucleocapsid
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-mCherry::LANS1

   Plasmid

   #60784

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS1
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. SL2-sgTelo-MTSa/BFP/pdCas9-C1

   Plasmid

   #162760

   Purpose

   Expressing dCas9 and sgRNA containg MTSa targeting telomeres

   Depositor

   Antony K Chen

   Article

   Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

   Insert

   dCas9 and sgRNA(SL2-sgTelo-MTSa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9(nuclease deactivated Cas9)

   Promoter

   U6/CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RFN_EGFP_81

   Plasmid

   #60073

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R, R883K, R887A

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

    Plasmid

    #170466

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA_DiCre_59.F2

    Plasmid

    #60207

    Purpose

    Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
    

    Use

    Tags

    FKBP12 and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 19-59 of the humanized version of Cre…

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. SL2-sgSat-MTSb/BFP/pdCas9-C1

    Plasmid

    #162761

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting centromeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgSat-MTSb)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDN43

    Plasmid

    #61346

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS11 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEx-mCherry-LINXb3

    Plasmid

    #73615

    Purpose

    Light-induced nuclear export switch for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXb3
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP8GF-AtTIR1

    Plasmid

    #188427

    Purpose

    Arabidopsis Nuclear Auxin Signaling Receptor TIR1

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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