We narrowed to 6,084 results for: nls

1. ELK1 B box-NLS pcDNA3.1/HisC

   Plasmid

   #121095

   Purpose

   Expression of the B box fragment of ELK1

   Depositor

   Paul Herring

   Article

   Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Tags

   6XHis-Omni-xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

   Plasmid

   #107303

   Purpose

   Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   ZFP_VEGFA-TS2
   

   Use

   CRISPR

   Tags

   2xNES, 3x Flag, 2xNLS, and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

   Plasmid

   #107304

   Purpose

   Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   ZFP_VEGFA-TS3
   

   Use

   CRISPR

   Tags

   2xNES, 3x Flag, 2xNLS, and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV7.1-Flag-NLS-hSPDEF delta2

   Plasmid

   #100871

   Purpose

   Mammalian expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 71-246aa

   Promoter

   CMV

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINDUCER20-Flag-NLS-hSPDEF delta3

   Plasmid

   #100876

   Purpose

   Lentiviral expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 243-335aa

   Promoter

   TRE2

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV7.1-Flag-NLS-hSPDEF delta3

   Plasmid

   #100872

   Purpose

   Mammalian expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 244-335aa

   Promoter

   CMV

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV7.1-Flag-NLS-hSPDEF delta1

   Plasmid

   #100870

   Purpose

   Mammalian expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 1-248aa

   Promoter

   CMV

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

   Plasmid

   #68276

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS(4E) mCherry
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   RPS2

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC-Flag-CHES1L-DBD-NLS

   Plasmid

   #51841

   Purpose

   Flag-CHES1L mutant encoding the DNA binding and NLS domains only

   Depositor

   Gerardo Ferbeyre

   Article

   Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

   Insert

   CHES1 (FOXN3 Human)
   

   Use

   Retroviral

   Tags

   Flag and NLS

   Expression

   Mutation

   contains CHES1 amino acids 110-205

   Promoter

   Available since

   July 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. SPL11p::pCGTAG (SPL11p::NLS-GFP)

    Plasmid

    #27411

    Depositor

    David Bartel

    Article

    Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

    Insert

    SPL11 promoter (AT1G27360 Mustard Weed)
    

    Use

    Tags

    Expression

    Mutation

    SPL11 promoter transcriptionally fused to a nucle…

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

    Plasmid

    #115141

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Orf2mor anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid

    #119554

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL6puro-JeT-pmRevErbA-mScaI3-NLS-Pest

    Plasmid

    #240107

    Purpose

    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    added JeT Promoter

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pL6puro-JeT-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240106

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    added JeT Promoter

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240112

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    added SV40-Enhancer

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-Pest

    Plasmid

    #240116

    Purpose

    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    added SV40-Enhancer

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest

    Plasmid

    #240111

    Purpose

    A lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    added JeT Promoter

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

    Plasmid

    #118617

    Purpose

    Lentiviral expression of mTurq2 tagged PCNA

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    TK-NLS-mTurq2-PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mTurq2

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid

    #155305

    Purpose

    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    

    Use

    Lentiviral

    Tags

    2xNLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

    Plasmid

    #31476

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV d14 GFP donor EF1s I-SceI
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    GFP has 14 amino acids deleted from c terminus

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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