We narrowed to 18,759 results for: nls
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Plasmid#191795Purposevector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6DepositorInserthumanized hfCas13d
UseAAV and CRISPRExpressionMammalianMutationA134V, A140V, A141V, A143VPromoterEFS, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-SasgRNA scaffold-CMV-nSaCas9
Plasmid#171695PurposeExpresses nSaCas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA scaffold-nSaCas9-NLS
UseCRISPRExpressionMammalianMutationD10APromoterU6 and CMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianPromoterCbhAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EmptyTranscriptionalReporter
Plasmid#62453PurposeMXS_chaining vector with 2 cassettesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA
UseSynthetic BiologyAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-HA-NEK7
Plasmid#75142PurposeExpresses N-terminal HA-tagged NEK7 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN_TT mCh-V5-NES-EGFP-SV40 NLS
Plasmid#192318PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-SV40 NLSDepositorInsertmCh-V5-NES-EGFP-SV40 NLS
UseGateway CloningTags3xFLAGAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-DEVD-mCardinal-NLS
Plasmid#164052PurposeFluorescent reporter for caspase-3DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-mCardinal-NLS
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mini-donor - NKX2.5-nEGFP
Plasmid#209287Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-nls
UseKnockin donor vectorAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_TRE-->2xNLS_IFP_PGK_PuroR
Plasmid#162238PurposeLentiviral vector for the expression of TRE-->2xNLS_IFP_PGK_PuroR in mammalian cellsDepositorInsertNLS IFP
UseLentiviralExpressionMammalianAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFv-nosP-Cas9
Plasmid#138402PurposeExpresses Cas9 in the germline of DrosophilaDepositorInsertCas9-NLS
ExpressionInsectPromoterUASAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT_HaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2
Plasmid#254206PurposeMamalian cell expression of nuclear HaloTag7-CLIP-tag2 and nuclear mTurquoise2DepositorPublicationInsertHaloTag7-CLIP-tag2-NLS-P2A-NLS-mTurquoise2
ExpressionMammalianPromoterCMVAvailable sinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
CAG-mKate2-NLS
Plasmid#248044PurposeEncodes nuclear mKate2 fluorescent protein, under control of the CAG promoter. The construct is flanked by Tol2 sites for transgenesis.DepositorInsertmKate2-NLS
ExpressionBacterialMutationmKate2 was derived from mKate with the following …PromoterCAGAvailable sinceMay 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
ACE16-NLS
Plasmid#210727PurposeNIR light-activated intein-based gene expression system (C-part)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACE16-NLS
ExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-CanlonicSF
Plasmid#188390PurposeTo explore lactate dynamics in intact systemsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-CanlonicSF
TagsNLS and mycExpressionMammalianPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMEH295 NLS dTomato 1x wild type MS2 loop
Plasmid#168214PurposeMammalian expression of nuclear-localized dTomato with 1 wild type MS2 loopDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 RPEL1-EGFP-3XNLS
Plasmid#58469PurposeExpresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Frb-NLS-mCherry
Plasmid#217613Purposepart B of SPARK-OFFDepositorInsertFrb-NLS-mCherry
UseLentiviralExpressionMammalianAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
SBE-NLS-mCherry PGK-rtTA3
Plasmid#195332PurposeLentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3DepositorInsertsNLS-mCherry
rtTA3
UseLentiviral and RNAiExpressionMammalianPromoterhPGK and miniCMVAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only