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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GCaMP6f

Plasmid

#135632

Purpose

AAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSP72 GSK3beta

Plasmid

#60125

Purpose

for transcription of GSK3beta

Depositor

Andrew McMahon

Insert

Glycogen synthase kinase 3 beta (Gsk3b Mouse)

Use

Cloning

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_SEPT2

Plasmid

#71547

Purpose

mammalian expression of Sept 2 with a GFP fusion

Depositor

Elias Spiliotis

Article

Bowen et al J Cell Biol. 2011 Jul 25;194(2):187-97.

Insert

Septin 2 (Septin2 Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-Tcl1

Plasmid

#13364

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

T-cell lymphoma breakpoint 1 (Tcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hysn-flex-dsRed-shscramble

Plasmid

#71383

Purpose

Expresses scramble shRNA Cre-dependently by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

human synapsin promoter-flex switch-dsRed-shscramble

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

human synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA1 TKIT

Plasmid

#169441

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Pom121 GFP

Plasmid

#187680

Purpose

In vivo visualization of NPC protein Pom121

Depositor

Mary Beckerle

Insert

Pom121 (Pom121 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Fos

Plasmid

#131595

Purpose

expression of mouse Fos in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Fos (Fos Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MPZ(P0)promoter pGL3-TATA

Plasmid

#21259

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero promoter (Mpz Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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CBIG-RORb

Plasmid

#48709

Purpose

bicistronic expression of RORb and EGFP

Depositor

Jeffrey Macklis

Insert

RORb (Rorb Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

CMV/beta-actin

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Tags

palmitoylated EGFP

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrascomG12D

Plasmid

#206844

Purpose

To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Codons altered to most optimum based on mouse cod…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

Plasmid

#86233

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-2 [L107/39R]

Plasmid

#177452

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-2 (Mouse). Derived from hybridoma L107/39.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Neuroligin-2 (Mus musculus) recombinant mouse monoclonal antibody (Nlgn2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-SSTR3-N-17

Plasmid

#55135

Purpose

Localization: Cilium, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

SSTR3

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2B -1538/+64

Plasmid

#24942

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s pc3901

Plasmid

#201700

Purpose

Expresses of GFP-tagged mTet1s in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK1

Plasmid

#122672

Purpose

Expresses mouse PCSK1 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

proprotein convertase subtilisin/kexin type 1 (PCSK1) (Pcsk1 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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