We narrowed to 2,911 results for: PPL

1. pIVEX2.3-CS2-3c-ON8-EGFP

   Plasmid

   #228453

   Purpose

   LS12-responsive riboswitch

   Depositor

   Keisuke Fukunaga

   Article

   Fukunaga et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

   Insert

   CS2-3c-ON8 riboswitch-regulated EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmirGLO-mir302-8mer4x (pEZ185)

   Plasmid

   #229059

   Purpose

   Dual-luciferase reporter containing 4 copies of the 8mer site of human miR-302a

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   Human miR-302a binding sites
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI5-CasRx-NLS-HA (pKW919)

   Plasmid

   #229047

   Purpose

   Mammalian expression of CasRx-NLS-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCI5-CasRx-NES-HA (pKW387)

   Plasmid

   #229048

   Purpose

   Mammalian expression of CasRx-NES-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   NES-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-DEST (JDW 1205)

   Plasmid

   #229821

   Purpose

   A PiggyBac destination vector compatible with gateway cloning system.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pB-Neo-DEST (JDW 940)

   Plasmid

   #229825

   Purpose

   A neo selectable, PiggyBac destination vector compatible with gateway cloning system.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-phiC31o (JDW 453)

   Plasmid

   #229842

   Purpose

   A CMV driven Phi31 recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   phiC31 codon optimized, PhiC31o
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEM_ΔndhD2.km

   Plasmid

   #185532

   Purpose

   a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

   Depositor

   Paula Tamagnini

   Article

   Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

   Insert

   nptII gene flanked by ndhD2 upstream and downstream regions
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEM_Δflv3.sm

   Plasmid

   #185533

   Purpose

   a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

   Depositor

   Paula Tamagnini

   Article

   Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

   Insert

   aadA gene flanked by flv3 upstream and downstream regions
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYAMTr2GCsgSeLEU2

    Plasmid

    #224870

    Purpose

    Disruption of S. eubayanus type LEU2 genes

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYAMTrGCsgScLEU2

    Plasmid

    #224869

    Purpose

    Disruption of S. cerevisiae type LEU2 genes

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYAMTr2GCsgScLEU2

    Plasmid

    #224868

    Purpose

    Disruption of S. cerevisiae type LEU2 gene

    Depositor

    Katsunori Suzuki

    Article

    Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

    Insert

    pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJMP8835

    Plasmid

    #220902

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJMP8921

    Plasmid

    #220903

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJMP8923

    Plasmid

    #220904

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP8932

    Plasmid

    #220906

    Purpose

    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-V5-mTagBFP2 (JDW 871)

    Plasmid

    #224531

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLPP4

    Plasmid

    #209966

    Purpose

    Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P40)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLP-EV

    Plasmid

    #209973

    Purpose

    Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    CDS (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLPC53

    Plasmid

    #209962

    Purpose

    Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    5' Connector (5C1CSN_Tcat+sai)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge