We narrowed to 3,229 results for: PPL

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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scAAV-hSyn-Cre

Plasmid

#138253

Purpose

A self-complementary AAV vector expresses Cre recombinase from hSyn promoter.

Depositor

Li Zhang

Article

Zingg et al J Neurosci. 2020 Apr 15;40(16):3250-3267

Insert

Cre recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAXY0001

Plasmid

#179834

Purpose

Binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Expression

Plant

Promoter

35S

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS1-gfp

Plasmid

#109288

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

Multiple Cloning Site and GFP

Expression

Bacterial

Promoter

Kanamycin

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pST-2K

Plasmid

#44564

Purpose

for ectopic constitutive expression of two mycobacterial proteins in one vector

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EBFP2

Plasmid

#167889

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro

Plasmid

#167911

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-R-GECO1.2

Plasmid

#45494

Purpose

Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

R-GECO1.2

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCT5-bac1.8

Plasmid

#119871

Purpose

Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Article

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR'
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

SNP in 592 bp

Promoter

PserA promoter and Pveg promoter with CuO sequence

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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PB-CAG-GFPd2

Plasmid

#115665

Purpose

Piggybac transposon plasmid for destabilized eGFP (GFPd2)

Depositor

Jordan Green

Article

Rui et al ACS Appl Mater Interfaces. 2019 Feb 22.

Insert

GFPd2

Use

Synthetic Biology

Tags

Destabilization

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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p458 VRER

Plasmid

#101728

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pminiTol2-5xUAS-hsp-sfGFP-zP2A-zN2cG-zP2A-TVA (UGNT)

Plasmid

#240273

Purpose

Helper plasmid for zebrafish rabies virus based-circuit tracing

Depositor

Xufei Du

Article

Chen et al eLife 13:RP100880

Inserts

N2cG (codon-optimized for Danio rerio)
sfGFP (codon-optimized for Danio rerio)
TVA (codon-optimized for Danio rerio)

Use

Zebrafish expression

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pSCARS1

Plasmid

#174868

Purpose

Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

Depositor

Zengyi Shao

Article

Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

Insert

ARS1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA0650

Plasmid

#240487

Purpose

CRISPRi-mediated Knockdown of RPA0650

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-O-GECO1

Plasmid

#46025

Purpose

Orange intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

O-GECO1

Expression

Mammalian

Mutation

R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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HA.PKD.S738E/S742E

Plasmid

#10810

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

Activation loop active: S738E and S742E (bp 2212-…

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pCas9

Plasmid

#167547

Purpose

Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

Depositor

Howard Hang

Article

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Inserts

chloramphenicol acetyl transferase
pUC19 origin of replication

Use

CRISPR

Expression

Bacterial

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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