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pMOD4 galK-GT

Plasmid

#19183

Depositor

Al Fisher

Publication

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

galK (galK E.coli)

Use

Recombineering

Tags

50 nucleotides from N-term TAP, C-term TAP, and F…

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-WT-WLS-HA-IRES-GFP

Plasmid

#178061

Purpose

Expression of WLS-HA protein (WT)

Depositor

Joseph Gleeson

Publication

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-DCA-muGFP

Plasmid

#166727

Purpose

AAV, encodes monomeric ultrastable GFP (muGFP)

Depositor

Daniel Scott

Publication

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Insert

muGFP

Use

AAV

Promoter

Chicken Beta Actin

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1595

Plasmid

#121062

Purpose

mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mKate2^SEC^BioTag::AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

Expression

Worm

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSF11-wmTagBFP2

Plasmid

#184934

Purpose

Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mTagBFP2

Expression

Worm

Promoter

tag-168

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

Plasmid

#127355

Purpose

gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

Depositor

Dominique Bergmann

Publication

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and mVenus

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTXRE5-Z

Plasmid

#112509

Purpose

Reporter plasmid, responsive to activated Ah receptor/Arnt

Depositor

Charles Miller

Publication

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

RW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)

Expression

Yeast

Promoter

5 XREs+ minimal Cytochrome C

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-NHERF2

Plasmid

#28292

Depositor

Maria-Magdalena Georgescu

Publication

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF2 (NHERF2 Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Publication

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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L2534

Plasmid

#1608

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, IVSA, rf2128, IVSM, IVSL, dec…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLexA::lis-1

Plasmid

#11338

Depositor

Guy Caldwell

Insert

lis-1 (lis-1 Nematode)

Tags

LexA DNA Binding Domain

Expression

Yeast

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-LifeAct-Venus

Plasmid

#87613

Purpose

Actin-targeted Venus (YFP).

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-Venus

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GST-AR-1-660

Plasmid

#104196

Purpose

Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Publication

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-660 (AR Human)

Tags

GST

Expression

Bacterial

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2139

Plasmid

#74111

Purpose

Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG

Use

Gateway topo cloning

Tags

PH

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLexA::nud-1

Plasmid

#11339

Depositor

Guy Caldwell

Insert

nud-1 (nud-1 Nematode)

Tags

LexA DNA Binding Domain

Expression

Yeast

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-CAML-Myc-His

Plasmid

#22295

Depositor

Erik Lillehoj

Publication

Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

Insert

calcium modulating ligand (CAMLG Human)

Tags

Myc-His

Expression

Mammalian

Available since

Dec. 2, 2009

Availability

Academic Institutions and Nonprofits only
pLVX-NPC1(P691S)-FLAG

Plasmid

#164973

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Publication

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P691S

Promoter

EF1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Publication

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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