166,169 results

1. [#GS16-LV] Syn1-MitoTRACER - Donor

   Plasmid

   #233565

   Purpose

   Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

   Depositor

   Simon Grelet

   Article

   Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

   Insert

   MitoTRACER-Donor
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   GFP11 and HA Tag

   Expression

   Mammalian

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Sport6-CD81-pHluorin

   Plasmid

   #130903

   Purpose

   Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD81-pHluorin (CD81 Human, aequorea victoria)
   

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   pHluorin inserted between Leu-49 and Gly-50 in ex…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-DEST53 PINK1 N-GFP

   Plasmid

   #13315

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xNFAT-ZsG-hCD8

   Plasmid

   #153417

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. UBC-4xmNG-dMCP

   Plasmid

   #231093

   Purpose

   Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).

   Depositor

   John Ngo

   Article

   Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

   Insert

   4xmNG-dMCP
   

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-N1 GBP (GBP-mCherry)

   Plasmid

   #162879

   Purpose

   Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   GFP Binding Protein
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTNS2

   Plasmid

   #64968

   Purpose

   Tn7 transposase expression

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   tnsABCD
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCAG-HIF1 alpha K532R

   Plasmid

   #21102

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

9. Nuclear PancACe

   Plasmid

   #215706

   Purpose

   Expresses acetyl-CoA biosensor in the nucleus

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19

    Plasmid

    #50005

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cloning vector

    Promoter

    lac

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2B-GFP

    Plasmid

    #11680

    Depositor

    Geoff Wahl

    Article

    Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

    Insert

    Human histone H2B (H2BC11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. CDH5-AbR-GFP

    Plasmid

    #122970

    Purpose

    Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

    Depositor

    Budd A. Tucker

    Inserts

    CDH5p
    CopGFP
    Zeocin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209723

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MCS-BioID2-HA

    Plasmid

    #74224

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lentiviral CaMKKbeta

    Plasmid

    #33322

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    human PGK

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lentiviral CaMKKbeta K193A

    Plasmid

    #33323

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    K194A

    Promoter

    human PGK

    Available Since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. psfTn5

    Plasmid

    #79107

    Purpose

    bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

    Depositor

    Sten Linnarsson

    Insert

    transposase 5
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E54K and L372P

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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