Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,584 results

Showing: 1421 - 1440 of 163584 results

  2. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid
    #107581
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107581-plasmid-map-sequence-id-209155

    Enlarge

  5. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

    Enlarge

  6. pKSJ376

    Plasmid
    #103065
    Purpose
    Expresses colicin E1 with R domain deletion and ImmE1
    Depositor
    Karen Jakes, Alan Finkelstein
    Article
    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0
    Inserts
    colicin E1 immunity protein (pColE1_26, imm E. coli)
    cea/colicin E1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R (receptor-binding domain) deletion in colicin E…
    Promoter
    T7 lac (from pET) and T7 lac (from pET52-b)
    Available Since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    103065-plasmid-map-sequence-id-200637

    Enlarge

  10. pCMV-iGlu3Fast

    Plasmid
    #226500
    Purpose
    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release
    Depositor
    Katalin Torok
    Article
    Tran et al bioRxiv, August 30th, 2024
    Insert
    iGluSnFR3 S72T
    Use
    Tags
    IgK, Myc tag, and PDGFR Beta TM domain
    Expression
    Mammalian
    Mutation
    S72T
    Promoter
    CMV
    Available Since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    226500-plasmid-map-sequence-id-448688

    Enlarge

  11. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

    Enlarge

  12. HPSE-bio-His

    Plasmid
    #53407
    Purpose
    Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    HPSE (HPSE Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53407-plasmid-map-sequence-id-83186

    Enlarge

  15. pHBintT

    Plasmid
    #48134
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Article
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Oct. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    48134-plasmid-map-sequence-id-69938

    Enlarge

  16. BII-tdT-inv-GFP

    Plasmid
    #133393
    Purpose
    Piggybac vector for bidirectional promoter reporter assay
    Depositor
    Blair Madison
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    No promoter
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133393-plasmid-map-sequence-id-268036

    Enlarge

Showing: 1421 - 1440 of 163584 results