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pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Publication

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Publication

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Publication

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLifeAct_mScarlet_N1

Plasmid

#85054

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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mCherry-CD9-10

Plasmid

#55013

Purpose

Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

CD9 (CD9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-MCS2

Plasmid

#46954

Purpose

Cloning plasmid for the generation of Knock-In in human cells using rAAV.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Use

AAV

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Mfn1-Myc

Plasmid

#23212

Depositor

David Chan

Publication

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn1 (Mfn1 Mouse)

Tags

6xHis, 9xMyc, and Myc

Expression

Mammalian

Promoter

CMV

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pmAmetrine-DEVD-tdTomato

Plasmid

#18879

Purpose

Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

Depositor

Robert Campbell

Publication

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)

Expression

Mammalian

Mutation

mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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M38 TOP-dGFP

Plasmid

#17114

Depositor

Randall Moon

Insert

Tcf/Lef sites (Ctnnb1 )

Tags

GFP

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3-TLR3-CFP

Plasmid

#13641

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 3 (TLR3 Human)

Tags

CFP

Expression

Mammalian

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Publication

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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HA-FOXO3a TM

Plasmid

#1788

Depositor

Michael Greenberg

Publication

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A (no longer detectably phosphor…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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p6XHis-T7(P266L)

Plasmid

#174866

Purpose

Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

Depositor

Anna Pyle

Publication

Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

Insert

T7 RNA polymerase

Tags

6XHis-tag

Expression

Bacterial

Mutation

P266L

Promoter

lac promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGP-CMV-jGCaMP8m

Plasmid

#162372

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pACRISPR

Plasmid

#113348

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Publication

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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PL-SIN-Nanog-EGFP

Plasmid

#21321

Purpose

Lentiviral vector, expresses EGFP under Nanog promoter

Depositor

James Ellis

Publication

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Nanog promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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