4,202 results
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Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_gRNA-Puro
Plasmid#84752PurposeExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoterDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry
Plasmid#114472PurposemCherry ControlDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVPromoterhSynAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
Plasmid#20945Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralExpressionMammalianMutationH134R mutation in ChR2PromoterhSynAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-GW
Plasmid#60323PurposeThis is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).DepositorTypeEmpty backboneUseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-LC3
Plasmid#21075PurposeMammalian expression of rat LC3 fused to mRFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
TagsmRFPExpressionMammalianMutationFirst ATG is not included.Available SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-SREBP-1a
Plasmid#26801DepositorAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Zsgreen
Plasmid#18121DepositorInsertZsgreen
UseLentiviralExpressionMammalianAvailable SinceFeb. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-DsRed
Plasmid#13769PurposeCre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertDsRed2
UseCre/Lox; Flp/frtExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE GFP
Plasmid#10668PurposeEmpty backbone for retroviral gene expression. Select recipient cells using GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-Q63L
Plasmid#12968DepositorInsertRhoA constitutively active (RHOA Human)
TagsEGFPExpressionMammalianMutationQ63L Constitutively activeAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMA4008
Plasmid#70109PurposemtDNA destruction/production of rho-0 cellsDepositorInsertsEGFP
Herpes simplex virus UL12.5M185
TagsnoneExpressionMammalianMutationN-terminal deletion of UL12.5PromoterCMV and RSVAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-C1
Plasmid#54759PurposeLocalization: C1 Cloning Vector, Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsmEGFPExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-19b_MMLV-RT
Plasmid#153312PurposeBacterial expression of MMLV reverse transcriptaseDepositorInsertMMLV Reverse Transcriptase
Tags10x HisExpressionBacterialAvailable SinceJune 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-HA-UTX
Plasmid#24168PurposeMammalian expression of human UTX with HA tagDepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
SIRT6 Flag
Plasmid#13817PurposeMammalian expression of human SIRT6 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloPromoterSFFVAvailable SinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only