162,676 results

1. pAAV-MCS2

   Plasmid

   #46954

   Purpose

   Cloning plasmid for the generation of Knock-In in human cells using rAAV.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIB-HIS-TEV-scCAK1

   Plasmid

   #177014

   Purpose

   Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. gag-COM-pol

   Plasmid

   #211373

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-COM-pol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

   Plasmid

   #85583

   Purpose

   cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

   Depositor

   Paul Sternberg

   Article

   Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   Prab-3-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Prab-3-GAL4-SK(DBD)-VP64

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SBE4-Luc

   Plasmid

   #16495

   Purpose

   Luciferase reporter containing four copies of the Smad binding site

   Depositor

   Bert Vogelstein

   Article

   Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

   Insert

   Smad binding element
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. BRCA1 A10.2 gRNA

   Plasmid

   #90549

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA1 (Guide Designation A10.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. DDX5

   Plasmid

   #155523

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-PVX

   Plasmid

   #213040

   Purpose

   PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-hROSA26-1-1kbdonor

    Plasmid

    #234736

    Purpose

    PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    hROSA26-1 crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

    Plasmid

    #235576

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    William Hahn, David Root, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-Cre

    Plasmid

    #55636

    Purpose

    Expresses Cre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV11

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSANG10-3F-BG4

    Plasmid

    #55756

    Purpose

    Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

    Depositor

    Shankar Balasubramanian

    Article

    Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

    Insert

    BG4
    

    Use

    Tags

    6xHis, pelB leader, and tri-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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