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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 30,488 results for: ica

Showing: 2131 - 2160 of 30488 results

  1. pAAV_GR-RE_MLPmin_BC1093-luc2

    Plasmid
    #227113
    Purpose
    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093
    Depositor
    Michael Wehr
    Article
    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523
    Insert
    12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227113-plasmid-map-sequence-id-454408

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  3. pRSF-G1TMSNKRS

    Plasmid
    #198323
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198323-plasmid-map-sequence-id-393448

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  4. pCapVQ329R

    Plasmid
    #183029
    Purpose
    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase variant CapVQ329R
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    glutamine 329 changed to arginine
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183029-plasmid-map-sequence-id-361616

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  5. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid
    #176245
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176245-plasmid-map-sequence-id-346567

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  6. pNOC_hfnCas12a_crRNA NR2

    Plasmid
    #176248
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176248-plasmid-map-sequence-id-347016

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  7. pNOC_hfnCas12a_crRNA NR1

    Plasmid
    #176247
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176247-plasmid-map-sequence-id-346571

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  8. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid
    #176246
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176246-plasmid-map-sequence-id-346569

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  9. pNOC_hfnCas12a_crRNA NR3

    Plasmid
    #176249
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    176249-plasmid-map-sequence-id-347037

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  19. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Article
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway cloning
    Tags
    SspB and mCherry
    Expression
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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  23. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid
    #159107
    Purpose
    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).
    Depositor
    Thomas Tuschl
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    NSP12
    Use
    Tags
    His-SUMO tag
    Expression
    Bacterial
    Mutation
    None
    Promoter
    T7
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    159107-plasmid-map-sequence-id-310025

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  24. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Article
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159390-plasmid-map-sequence-id-310625

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  29. FLINC-AKAR1-CAAX

    Plasmid
    #87705
    Purpose
    Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    FLINC-AKAR1-CAAX
    Use
    Tags
    C-terminal targeting sequence from Kras
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87705-plasmid-map-sequence-id-176711

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Showing: 2131 - 2160 of 30488 results