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pREDIT_MCP-RecT

Plasmid

#164803

Purpose

mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Insert

RecT

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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Venus-BclXL-pEGFP-C1

Plasmid

#177409

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

Bcl-XL

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPI227

Plasmid

#113231

Purpose

safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

LifeAct

Use

Safe haven knock-in plasmid for the act5 locus,li…

Tags

GFP

Promoter

act5

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAB076i7

Plasmid

#79201

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry::LANS4

Plasmid

#60785

Purpose

Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

Depositor

Brian Kuhlman

Article

Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

Insert

LANS4

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAB107a

Plasmid

#79218

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-P2A-GFP

Plasmid

#247161

Purpose

In vitro transcription of human codon optimized wildtype Dn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-SopE

Plasmid

#221327

Purpose

Dox-inducible expression of SopE in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

SopE

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-dCas9-KOX1

Plasmid

#240528

Purpose

Expresses MMLVgag–dCas9-KOX1 for producing RENDER-dCas9-KOX1

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-dCas9-KOX1

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-OPHN1

Plasmid

#221310

Purpose

Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

OPHN1 (pLxIS) (Ophn1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

Plasmid

#185918

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Insert

human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBS FGF3 isoform 1

Plasmid

#19230

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

Plasmid

#172065

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-nef-CIAO2-TVA-mCherry

Plasmid

#149297

Purpose

Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

TVA:mCherry

Use

AAV

Mutation

Start codon removed.

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAB076i6

Plasmid

#79200

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (no operator); Ppro1

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/hGGA3(GAT)

Plasmid

#79436

Purpose

Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

Depositor

Kazuhisa Nakayama

Article

Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

Insert

GGA3 (GGA3 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 107-286

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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p6610 MSCV-IP N-HAonly TIMP1

Plasmid

#35009

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

TIMP1 (TIMP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-SYNDAPIN2

Plasmid

#221311

Purpose

Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-D3A-3L-dCas9

Plasmid

#240530

Purpose

Expresses MMLVgag–DNMT3A-3L-dCas9 for producing RENDER-DNMT3A-3L-dCas9

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-D3A-3L-dCas9

Tags

FLAG, HA

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Expression

Bacterial

Promoter

TetR-PT5-3

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJC.15A.sgRNA.TA

Plasmid

#232483

Purpose

sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Inserts

Toxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry

Use

CRISPR

Expression

Bacterial

Promoter

P3 constitutive promoter and native promoter

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Expression

Bacterial

Available Since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJW6.01

Plasmid

#62882

Purpose

Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

GFP::MEG-3

Tags

GFP

Expression

Worm

Promoter

meg-3

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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