We narrowed to 5,621 results for: pcas

1. pspCas9-POLI-ST2-com vector

   Plasmid

   #176234

   Purpose

   Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG ERT2-Cre-ERT2 lox

   Plasmid

   #183447

   Purpose

   CreOFF tamoxifen-inducible Cre

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   ERT2-Cre-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mCherry-FKBP-GEF(TIAM1)

   Plasmid

   #85156

   Purpose

   acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mEGFP-CaMKIIa (T305D/T306D)

   Plasmid

   #127611

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 305/306 to Alanine

   Promoter

   pCAG

   Available since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-rat PKA-RIIbeta-mEGFP

   Plasmid

   #45530

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RII beta tagged by mEGFP
   

   Use

   With chicken beta actin promoter and wpre

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA27-pHR-MiniCMV-TriTag (mTagBFP)

   Plasmid

   #199458

   Purpose

   Expression of TriTag (mTagBFP) in mammalian cells

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   TriTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MiniCMV

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-Flag-eSpCas9 (without sgRNA)

   Plasmid

   #92354

   Purpose

   Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-Z-SpCas9-gRNA-repeat-polyT-11

   Plasmid

   #197781

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   gRNA repeat for U6 sgRNA assembly
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

    Plasmid

    #107858

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 2) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pspCas9-ABE-3'UTR-sgRNA-2xMS2

    Plasmid

    #132554

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xNLS-NLP-cMyc-cMyc enAspCas12a

    Plasmid

    #182127

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc enAspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Flag-Avi-ires-CHD5

    Plasmid

    #68878

    Purpose

    Transient mammalian expression of CHD5 for BirA tagging

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Tags

    FLAG/AVI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCALNL-eGFP-NT1-6-gix-6-NT1

    Plasmid

    #81204

    Purpose

    contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    gix-Neo-gix deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-mcherry-WIPI2d-cs-TEV-STREP

    Plasmid

    #178912

    Purpose

    Mammalian expression of human WIPI2d with N-terminal mCherry and C-terminal Strep

    Depositor

    James Hurley

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2d
    

    Use

    Tags

    C term strep and N term mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xNLS-NLP-cMyc-cMyc AspCas12a

    Plasmid

    #182122

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Venus-pA_U6-BbsI (pE057)

    Plasmid

    #149351

    Purpose

    Mammalian expression vector for Venus

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS/Daxx myc S502A/669A

    Plasmid

    #19283

    Depositor

    Philip Leder

    Article

    Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

    Insert

    Daxx (Daxx Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

20. -161+80pCalb2/E2F2/NRF-1(-64)-like

    Plasmid

    #66742

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    CGCG(-64) to TACA

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCAG-EGFP/RFP-miR-E1-1int

    Plasmid

    #19819

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 . 8():77.

    Insert

    miRNA
    

    Use

    Cre/Lox and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pCAG-BFP-pA_U6-BbsI (pE058)

    Plasmid

    #149352

    Purpose

    Mammalian expression vector for BFP

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. M11 Cardiac Actin Promoter pCarA

    Plasmid

    #17148

    Depositor

    Randall Moon

    Insert

    cardiac actin promoter (actc1 Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pCSDest2-SpCas9-NLS-3XHA-NLS

    Plasmid

    #69220

    Purpose

    Expresses wild type SpCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBT250_(pCA-G-intron(Neo)-tTA2)

    Plasmid

    #36876

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #125748

    Purpose

    sfGFP and iCreERT2 fused by GSAx9 linker

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBT241_(pCA-ATG-intron-tTA2)

    Plasmid

    #36875

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tTA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid

    #137859

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-LplA(AILR,36)-NRX3b D137A

    Plasmid

    #43920

    Depositor

    Alice Ting

    Article

    Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

    Insert

    AILR-LplA36-NRX3b (lplA Human, Synthetic)
    

    Use

    Tags

    2XHA

    Expression

    Mammalian

    Mutation

    D137A

    Promoter

    Chicken Beta Actin

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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