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We narrowed to 3,822 results for: pes

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lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

Plasmid

#176838

Purpose

To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

sgPDX1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-egfp-U3-UL8

Plasmid

#166694

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Rpb1-linker

Plasmid

#138468

Purpose

Expresses GST-tagged Rpb1 linker in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1460-1585

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-4xCTD

Plasmid

#138471

Purpose

Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

4x CTD repeat

Promoter

T5

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-mu24-ORF24-2xStrep

Plasmid

#138453

Purpose

Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

mu24-ORF24 (ORF24 MHV68/KSHV)

Use

Tags

Strep

Expression

Mammalian

Mutation

mu24 amino acids 1-191 fused to ORF24 amino acids…

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hADM-RFP

Plasmid

#22922

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human adrenomedullin promoter driving DsRedExpress (ADM Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pX330-Utf1 gRNA

Plasmid

#128842

Purpose

gRNA for targeting mouse Utf1 locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Utf1 gRNA (Utf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSFG-TFII-I-GFP Y248&249F

Plasmid

#22196

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines. …

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

Plasmid

#60393

Purpose

Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast, Synthetic)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Use

Tags

BirA

Expression

Bacterial

Mutation

G120C

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

Plasmid

#226577

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

I178A, L182A, L186A, V190A

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 WT

Plasmid

#222651

Purpose

For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Expression

Mutation

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Use

Tags

ALFA tag

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_MSX1-FKBP-mNeonGreen-V5

Plasmid

#215025

Purpose

AAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

msh homeobox 1 (MSX1 Human)

Use

AAV

Tags

FKBP-mNeonGreen-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT22gCfpUbiG4FER4P2A(#249)

Plasmid

#184070

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

Gal4bdFF-ERT2-P2A

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-U3-UL8

Plasmid

#166685

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA (UL29, UL8 HSV-1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_A37I-A44L

Plasmid

#162583

Purpose

Expresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

A37I and A44L

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X-ORF24-NTD-3L_A

Plasmid

#138466

Purpose

Expresses MBP-tagged ORF24-NTD 3L_A in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201; amino acids L73-75 mutated to …

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-2xCTD

Plasmid

#138470

Purpose

Expresses GST-tagged 2xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

2x CTD repeat

Promoter

T5

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A_L75A-3xFLAG

Plasmid

#138429

Purpose

Expresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73, L75 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L74A_L75A-3xFLAG

Plasmid

#138430

Purpose

Expresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L74, L75 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 R68A-3xFLAG

Plasmid

#138431

Purpose

Expresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid R68 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 R72A-3xFLAG

Plasmid

#138432

Purpose

Expresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid R72 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A_L74A-3xFLAG

Plasmid

#138433

Purpose

Expresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73, L74 mutated to alanine

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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