We narrowed to 2,364 results for: Efs

1. pMBP-AaCas12b

   Plasmid

   #113433

   Purpose

   Expresses Expresses 10xHis-MBP fused to AaCas12b

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AaCas12b
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMBP-LbCas12a D832A

   Plasmid

   #113517

   Purpose

   Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   D832A

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMBP-LbCas12a H759A

   Plasmid

   #113518

   Purpose

   Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   H759A

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-ER-rsTagRFP

   Plasmid

   #87620

   Purpose

   Endoplasmic reticulum (ER)-targeted rsTagRFP.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   ER-rsTagRFP
   

   Use

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMBP-LbCas12a

   Plasmid

   #113431

   Purpose

   Expresses 10xHis-MBP fused to LbCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Has Service

   Cloning Grade DNA

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Venus-CAAX

   Plasmid

   #87612

   Purpose

   Plasma membrane-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-LifeAct-Venus

   Plasmid

   #87613

   Purpose

   Actin-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   LifeAct-Venus
   

   Use

   Tags

   17-aa sequence from Abp140 (aka "Lifeact&quo…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FKBP-Venus-173-C

   Plasmid

   #87617

   Purpose

   FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   FKBP-Venus173-C
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TU#1450_unc-53a_cDNA

   Plasmid

   #82361

   Purpose

   express unc-53a cDNA

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   unc-53 a isoform (unc-53 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TU#1448_unc-73b_cDNA

    Plasmid

    #82362

    Purpose

    express unc-73b cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 b isoform (unc-73 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TU#1412_tiam-1_cDNA

    Plasmid

    #82364

    Purpose

    express tiam-1 cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    tiam-1 cDNA (tiam-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TU#1449_unc-73e_cDNA

    Plasmid

    #82363

    Purpose

    express unc-73e cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 e isoform (unc-73 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-FKBP-DMVF-181-C

    Plasmid

    #87611

    Purpose

    FKBP-tagged C-terminal Dronpa fragment for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    FKBP-DMVF181-C
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-4T1-RhoA G17A

    Plasmid

    #69357

    Purpose

    expression of nucleotide free RhoA in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G17A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-H2B-Venus

    Plasmid

    #87614

    Purpose

    Venus (YFP) fused to histone H2B.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    H2B-Venus (H2BC8 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T1-Cdc42 G15A

    Plasmid

    #69356

    Purpose

    expression of nucleotide free Cdc42 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-4T1-Rac1 G15A

    Plasmid

    #69355

    Purpose

    expression of nucleotide free Rac1 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-LifeAct-FRB-Venus-173-N

    Plasmid

    #87616

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-Venus173-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TU#1806_CRISPR_unc-73_exon21

    Plasmid

    #82360

    Purpose

    to create unc-73E null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Lyn-FRB-Venus-173-N

    Plasmid

    #87615

    Purpose

    FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Lyn-FRB-Venus173-N
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TU#1805_CRISPR_unc-73_exon2

    Plasmid

    #82359

    Purpose

    to create unc-73B null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Lyn-FRB-DMVF-181-N

    Plasmid

    #87609

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Lyn-FRB-DMVF181-N
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-LifeAct-FRB-DMVF-181-N

    Plasmid

    #87610

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-DMVF181-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lck-mTurquoise2-iLID

    Plasmid

    #176125

    Purpose

    Plasma membrane localized iLID

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    iLID
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HaloTag-3xrGBD

    Plasmid

    #176108

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-Lck-FRB-mTurquoise2

    Plasmid

    #176130

    Purpose

    Plasma membrane localized FRB, rapamycin heterodimerization

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    FRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-HaloAKAR2.1-CLC

    Plasmid

    #216492

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1-CLC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

    Plasmid

    #216495

    Purpose

    Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloEKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge