We narrowed to 15,034 results for: pET

1. pET-HJURP-CBP

   Plasmid

   #30393

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   HJURP CenpA Binding Domain (CENPA Budding Yeast, Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pET20b-delta133HtrA2 A141S

   Plasmid

   #16157

   Depositor

   L. Miguel Martins

   Article

   Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A141S

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. CyPet-A206K-PRL2

   Plasmid

   #149685

   Purpose

   For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

   Depositor

   Motoyuki Hattori

   Article

   Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

   Insert

   CyPet-A206K-PRL2
   

   Use

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   CyPet A206K

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28 Set9 mutant

   Plasmid

   #24083

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   H297A

   Promoter

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pET-28a(+)-FPPS

   Plasmid

   #108952

   Purpose

   heterologous expression of FPPS of G. stearothermophilus in E. coli

   Depositor

   Frank Schulz

   Article

   Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

   Insert

   Farnesyldiphosphat synthase (FPPS)
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-LaM4-1xTS

   Plasmid

   #162778

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   lac operator

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. TrmD_HaeIn/pET28b(+) N,C-tag

   Plasmid

   #12665

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. human TLNRD1 pET151

   Plasmid

   #159384

   Purpose

   Expresses human TLNRD1 in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His10-Src42a

    Plasmid

    #126674

    Purpose

    Expression of His10-Src42a for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Src42a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-21a(+)-IS200

    Plasmid

    #12604

    Depositor

    SeWon Suh

    Article

    Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

    Insert

    IS200 transposase (SSO1474 Sulfolobus solfataricus)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaG-24

    Plasmid

    #172750

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-24
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-p53 (Mouse 1-87)

    Plasmid

    #62061

    Purpose

    Expression of Mouse p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. C122-E16: CyPet

    Plasmid

    #162896

    Purpose

    Standard Gateway entry clone encoding CyPet fluorescent protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    CyPet fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET20b- hTPI Lys13Arg

    Plasmid

    #50726

    Purpose

    overexpression of the human TPI1 Lys13Arg allele in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    T7

    Available Since

    Feb. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-MBP-creM

    Plasmid

    #112238

    Purpose

    pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Insert

    CreM
    

    Use

    Tags

    MBP

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. D5 gene in PETCON vector

    Plasmid

    #87626

    Purpose

    expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

    Insert

    Designed allosteric Zn2+ and fluorescein binding antibody scFv D5
    

    Use

    Tags

    3x(G4S) Linker and cMyc tag on backbone

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET30Xa-SBL1(160S)

    Plasmid

    #66872

    Purpose

    Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
    

    Use

    Tags

    Histag from pET30Xa-LIC

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET30a BCP1 NTase

    Plasmid

    #195245

    Purpose

    Expresses phage BCP1 NTase

    Depositor

    Douglas Feldman

    Article

    Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

    Insert

    BCP1 phage NTase
    

    Use

    Tags

    His6 and SBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET30a-Av05 NTase

    Plasmid

    #195243

    Purpose

    Expresses phage Av05 NTase

    Depositor

    Douglas Feldman

    Article

    Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

    Insert

    phage Av05 NTase
    

    Use

    Tags

    His6 and SBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-BCA-Pep-H

    Plasmid

    #185782

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Pep-H
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBC2T-Ypet-FtnA

    Plasmid

    #108969

    Purpose

    expresses BC2-tag-Ypet-FtnA in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    FtnA (ftnA Escherichia coli)
    

    Use

    Tags

    BC2-tag and Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid

    #127637

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    YPet
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PP7 coat protein (PCP)

    Expression

    Bacterial

    Mutation

    Promoter

    pJ21306

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Promoter

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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