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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 24,890 results for: gfp

Showing: 3651 - 3700 of 24890 results

  6. EGFP–acVHH–PB

    Plasmid
    #169685
    Purpose
    An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody
    Use
    Tags
    modified STIM1-PB tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169685-plasmid-map-sequence-id-338140

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  8. SIN40C.EFS.eGFP.ARID3A-3'UTR-WT

    Plasmid
    #169297
    Purpose
    Constitutive overexpression of the 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
    Depositor
    Jan-Henning Klusmann
    Article
    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0
    Insert
    ARID3A-3'UTR-WT (ARID3A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169297-plasmid-map-sequence-id-337857

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  17. Nup54 no FG-mEGFP494(2)

    Plasmid
    #164271
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP
    Depositor
    Sanford Simon
    Article
    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164271-plasmid-map-sequence-id-325399

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  18. Nup54 no FG-mEGFP494(1)

    Plasmid
    #164270
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP
    Depositor
    Sanford Simon
    Article
    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164270-plasmid-map-sequence-id-325397

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  23. GFP-CD8a(7A)

    Plasmid
    #162498
    Purpose
    Expresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a with a mutated stem region (CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine and two serine residues are mu…
    Promoter
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162498-plasmid-map-sequence-id-323489

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  24. pCAG-mEGFP-HO-G

    Plasmid
    #164163
    Purpose
    Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal
    Depositor
    Isei Tanida
    Article
    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1
    Insert
    mEGFP A236K
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164163-plasmid-map-sequence-id-323957

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  25. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162504-plasmid-map-sequence-id-323646

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  26. ST-Tstem(5A)-GFP

    Plasmid
    #162502
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The mutated Tac stem region is inserted within ST…
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162502-plasmid-map-sequence-id-323507

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  27. ST-2xTstem-GFP

    Plasmid
    #162503
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162503-plasmid-map-sequence-id-323511

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  28. ST-Tstem-GFP

    Plasmid
    #162501
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The Tac stem region is inserted within ST6Gal1
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162501-plasmid-map-sequence-id-323506

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  29. Tac(5A)-GFP

    Plasmid
    #162495
    Purpose
    Expresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162495-plasmid-map-sequence-id-323475

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  30. GFP-Tac(5A)

    Plasmid
    #162493
    Purpose
    Expresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    Tac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162493-plasmid-map-sequence-id-323471

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  31. Nup54-mEGFP494(2)

    Plasmid
    #163428
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163428-plasmid-map-sequence-id-321665

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  32. pEGFP-rUSP19CYT

    Plasmid
    #155248
    Purpose
    for mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155248-plasmid-map-sequence-id-311946

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  33. pEGFP-rUSP19ER

    Plasmid
    #155247
    Purpose
    for mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) (Usp19 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155247-plasmid-map-sequence-id-311881

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  34. pLz26_CsoS2_N1-N2_GFP

    Plasmid
    #140851
    Purpose
    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CsoS2 NTD repeats 1-2 fused to GFP
    Use
    Tags
    Hexahistidine tag, Strep-Tag II, and Superfolder …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140851-plasmid-map-sequence-id-297981

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  36. pSB1C3-FeGFP

    Plasmid
    #138261
    Purpose
    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Insert
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138261-plasmid-map-sequence-id-273630

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Showing: 3651 - 3700 of 24890 results