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RCIscript-GoldyTALEN

Plasmid

#38142

Purpose

destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

Depositor

Daniel Carlson, Stephen Ekker

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

Insert

GoldyTALEN

Use

Mrna transcription vector

Tags

AcV5 and FokI homodimer

Mutation

Truncate at N and C terminous

Promoter

T3

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-H2A

Plasmid

#36207

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

H2A (H2AC16 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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MIGR1

Plasmid

#27490

Depositor

Warren Pear

Article

Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-3

Plasmid

#27289

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin beta 3 (ITGB3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM-3z/601

Plasmid

#26656

Depositor

Jonathan Widom

Article

Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

Insert

Expression

Bacterial

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti PGK V5-LUC Puro (w543-1)

Plasmid

#19360

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Skp2

Plasmid

#19947

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hKLF4

Plasmid

#20725

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLOX-Ttag-iresTK

Plasmid

#12246

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

SV40 Large T, HSV1-TK (SV40gp6 Simian virus)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Has Service

AAV8

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEBG

Plasmid

#22227

Purpose

GST fusion vector

Depositor

David Baltimore

Article

Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

Type

Empty backbone

Tags

GST

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pcDNA3

Plasmid

#18956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Article

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Available Since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR2-YFP

Plasmid

#13016

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 2 (TLR2 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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rAAV2/MG1.2

Plasmid

#184541

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-MG1.2 capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, Q588T; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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STAT3-GFP

Plasmid

#110495

Purpose

reporter of STAT3 signaling activity

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

4 repeats of STAT3 binding site M67 at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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