4,170 results

1. Lentiviral-TOP-dGFP-reporter

   Plasmid

   #14715

   Purpose

   Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   LEF-1/TCF-responsive promoter (Ctnnb1 )
   

   Use

   Lentiviral

   Tags

   d2 eGFP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHAC-mt-mKeima

   Plasmid

   #72342

   Purpose

   retrovirus construct for stable expressing mt-mKeima

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   mt-mKeima
   

   Use

   Retroviral

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. tFucci(SA)5

   Plasmid

   #153520

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(SA)5.
   

   Tags

   AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 12XCSL-d1EGFP

   Plasmid

   #47684

   Purpose

   Notch reporter

   Depositor

   Urban Lendahl

   Article

   Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

   Insert

   TP-1 promoter
   

   Use

   Notch reporter construct

   Tags

   d1EGFP

   Mutation

   hexamerized 50-bp EBNA2 response element of the T…

   Promoter

   NA

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-CMV-3xFLAG-gateway-EGFP

   Plasmid

   #122845

   Purpose

   N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MBP-FUS_FL_WT

   Plasmid

   #98651

   Purpose

   Expresses MBP-tagged full length FUS

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLAG-CMV-hErk1

   Plasmid

   #49328

   Purpose

   Expresses FLAG-tagged human Erk1

   Depositor

   Melanie Cobb

   Insert

   Erk1 (MAPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

   Plasmid

   #153004

   Purpose

   Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (N) (G78)
   

   Use

   Lentiviral

   Tags

   FKBP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBFP2-C1

    Plasmid

    #22880

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

    Insert

    SBFP2
    

    Expression

    Mammalian

    Available Since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-NR2B

    Plasmid

    #17925

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl D-aspartate receptor 2B (Grin2b Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1 beta-catenin WT

    Plasmid

    #16071

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    *

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. AICSDP-15:ACTB-mEGFP

    Plasmid

    #87425

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    March 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLKO.1 GFP shRNA

    Plasmid

    #30323

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    GFP shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR

    Plasmid

    #42875

    Purpose

    A crRNA expression plasmid for targeting a specific sequence.

    Depositor

    Luciano Marraffini

    Article

    Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    CRISPR-BsaI
    

    Use

    CRISPR; E.coli

    Expression

    Bacterial

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-cmyc

    Plasmid

    #16011

    Purpose

    mammalian expression of human cmyc

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

    Insert

    cmyc (MYC Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-p300

    Plasmid

    #23252

    Depositor

    Warner Greene

    Article

    Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

    Insert

    p300 (EP300 Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3XMEF2-luc

    Plasmid

    #32967

    Purpose

    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

    Depositor

    Ron Prywes

    Insert

    MEF2 binding sites (MEF2A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    MEF2

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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