We narrowed to 165,528 results for: addgene

1. pAAV-CAG-Lck-mTagBFP2

   Plasmid

   #234537

   Purpose

   to label the membrane of cells with a fluorescent protein (mTagBFP2)

   Depositor

   Hajime Hirase

   Article

   Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

   Insert

   Lck-mTagBFP2
   

   Use

   AAV

   Mutation

   None

   Promoter

   Short CAG

   Available Since

   June 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBHM2678

   Plasmid

   #241723

   Purpose

   Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BLE marker for selection on phleomycin

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBHM2680

   Plasmid

   #241725

   Purpose

   Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin S

   Depositor

   Hiten Madhani

   Article

   Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Sept. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MXS_BidirectionalCAG

   Plasmid

   #78175

   Purpose

   Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   Bidirectional CAG Promoter
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSADdeltaG-DsRed2

   Plasmid

   #32638

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   DsRed2
   

   Use

   Rabies virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-PERK(LD)-EGFP-HOTag3

   Plasmid

   #225678

   Purpose

   Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

   Depositor

   Likun Wang

   Article

   Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-mCherry

   Plasmid

   #217005

   Purpose

   lentiviral plasmid for gRNA cloning with selectable mCherry expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX302 Luciferase-V5 puro

    Plasmid

    #47553

    Purpose

    Lentiviral expression of V5-tagged Luciferase in mammalian cells

    Depositor

    Kevin Janes

    Article

    Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNR220

    Plasmid

    #79541

    Purpose

    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    TP901
    BxbI
    A118
    

    Tags

    His tag and LAA degradation tag

    Expression

    Bacterial

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti SpBsmBI sgRNA Puro

    Plasmid

    #62207

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVLINK-EF1alpha-5' NEXMIF

    Plasmid

    #236668

    Purpose

    5' AAVLINK plasmid for NEXMIF expression

    Depositor

    Zhonghua Lu

    Insert

    NEXMIF (NEXMIF Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB_EF1a_Gag-EIF4E

    Plasmid

    #212657

    Purpose

    piggyBac vector to express an MLV Gag-EIF4E fusion protein

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Piggybac

    Tags

    EIF4E

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHdzCas9-KRAB

    Plasmid

    #92341

    Purpose

    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

    Depositor

    I-Son Ng

    Article

    Kao et al Bioresour Technol. 2017 May 4. pii: S096

    Type

    Empty backbone

    Use

    CRISPR; Plant expression

    Tags

    KRAB

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CBh-DIO-EGFP

    Plasmid

    #87168

    Purpose

    Expression of eGFP in the presence of Cre

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    EGFP
    

    Use

    AAV

    Tags

    None

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSADdeltaG-GFP-F2

    Plasmid

    #32635

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    GFP
    

    Use

    Rabies virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-HypaCas9

    Plasmid

    #190604

    Purpose

    PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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