19,619 results

1. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-mVenus-p27K−

   Plasmid

   #176651

   Purpose

   It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

   Depositor

   Mohamed Bentires-Alj

   Article

   Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

   Insert

   mVenus-p27K- (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   mVenus

   Mutation

   Mutant K- (lacks affinity to Cdk)

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-EF1a ANXA11-mEmerald

   Plasmid

   #164210

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   EF1a

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-O-FUW-Neurod1

   Plasmid

   #30129

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   NeuroD1 (Neurod1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Golgi-7

   Plasmid

   #55052

   Purpose

   Localization: Golgi Complex, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Golgi (B4GALT1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE3G-MYCN

   Plasmid

   #104542

   Purpose

   PiggyBac vector encoding doxycycline-inducible human MYCN

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
   

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3G

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYIC

   Plasmid

   #18673

   Depositor

   Han Htun

   Article

   Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

   Type

   Empty backbone

   Tags

   3Myc-EYFP-HA-His6 and ECFP-HA-His6

   Expression

   Mammalian

   Available Since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1 CMYC

   Plasmid

   #164145

   Purpose

   Doxycycline inducible expression of c-MYC

   Depositor

   Richard Possemato

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Doxycycline inducible promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDae079 (eMutaT7[transition])

    Plasmid

    #187622

    Purpose

    Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Inserts

    TadA-8e-T7RNAP
    PmCDA1-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

    Plasmid

    #83842

    Purpose

    Fluorescent probe for S/G2 cell cycle transition and G2/M transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TetO-FUW-NICD

    Plasmid

    #61540

    Purpose

    Expresses NOTCH-ICD in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    NOTCH-ICD (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NOTCH intracellular domain (aa1761-2555); A1943S…

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB CAG-sfGFP

    Plasmid

    #133568

    Purpose

    CAG sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CMV-puro-2A-TEVp

    Plasmid

    #99610

    Purpose

    Lentivirus expressing puromycin resistance gene and TEV protease

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    Puromycin resistance (PAC gene)
    TEV protease
    

    Use

    Lentiviral

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115219

    Purpose

    Expresses PspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PspCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET3a-hUBA1

    Plasmid

    #63571

    Purpose

    Expression of human UBA1 tagged with His

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBA1 (UBA1 Human)
    

    Expression

    Bacterial

    Mutation

    Isoform 2 (1018 amino acids)

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMP31-1

    Plasmid

    #45905

    Depositor

    Harmit Malik

    Article

    Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

    Insert

    mitochondrial antiviral signaling protein (MAVS Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. integrin beta6 EGFP-N3

    Plasmid

    #13593

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189922

    Purpose

    AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e
    

    Use

    AAV

    Mutation

    SaCas9 D10A

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. EBFP2-Mito-7

    Plasmid

    #55248

    Purpose

    Localization: Mitochondria, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Mito (COX8A Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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