8,809 results

1. pRSET FlipSuc-90u delta1

   Plasmid

   #14475

   Depositor

   Wolf Frommer

   Article

   Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

   Insert

   FlipThuE W283A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   N192D, W283A, 13 amino acids deleted from linkers

   Promoter

   T7

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HsRCK

   Plasmid

   #25033

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   Rck (DDX6 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1036 pcDNA3 Myr HA Akt1

   Plasmid

   #9008

   Purpose

   Mammalian expression of human Akt1 with myr, HA tags

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   HA and myr

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyCatcher003

   Plasmid

   #133447

   Purpose

   Expresses SpyCatcher003 in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.3G

   Plasmid

   #14748

   Depositor

   Diane Mathis, Christophe Benoist

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeonGreen-2A-mTurquoise2

   Plasmid

   #98885

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mNeonGreen-2A-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Laconic/pcDNA3.1(-)

   Plasmid

   #44238

   Purpose

   a genetically-encoded sensor for lactate concentration, transport and metabolic flux

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

   Insert

   Laconic (LACtate Optical Nano Indicator from CECs)
   

   Use

   Lactate biosensor

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-RfxCas13d-HA

   Plasmid

   #141320

   Purpose

   Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   HA

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLionII

   Plasmid

   #1730

   Purpose

   This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

   Depositor

   Garry Nolan

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mScarlet-I-mTurquoise2

    Plasmid

    #98839

    Purpose

    Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mScarletI-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXG237

    Plasmid

    #141281

    Purpose

    ATF4 translational reporter

    Depositor

    Martin Kampmann

    Article

    Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

    Insert

    uORF1/2 of ATF4 followed by mApple
    

    Use

    Lentiviral

    Tags

    mApple

    Promoter

    CMV

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223-MET

    Plasmid

    #23889

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MET (MET Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVUT-tTR-KRAB

    Plasmid

    #11651

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Has Service

    Cloning Grade DNA

    Insert

    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-mRFP1

    Plasmid

    #21315

    Purpose

    MoMLV-based Retroviral vector, expresses mRFP1

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    monomeric Red Fluorescence Protein 1
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid

    #29659

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-NbALFA

    Plasmid

    #159987

    Purpose

    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mCherry-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mCherry and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Promoter

    T5-LacO

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. gag-COM-pol

    Plasmid

    #211373

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-COM-pol
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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