We narrowed to 13,947 results for: cas9

1. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

   Plasmid

   #197426

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

   Plasmid

   #199253

   Purpose

   Expression construct encoding the high specificity SpCas9-KA-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity S. pyogenes Cas9-D10A-KA nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. zCREST3:Cas9green; T2_gRNA

   Plasmid

   #197647

   Purpose

   pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   nrg1 type II sgRNA
   

   Use

   Tol2 destination vector

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. zCREST3:Cas9green; T1_gRNA

   Plasmid

   #199332

   Purpose

   pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   nrg1 type I sgRNA
   

   Use

   Tol2 destination vector

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-Cas9-sgHPRT1

   Plasmid

   #196713

   Purpose

   CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Insert

   Cas9-T2A-BSD-U6-sgHPRT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Promoter

   EF1a/hU6

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-CcuCas9

   Plasmid

   #192127

   Purpose

   Expresses CcuCas9

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   CcuCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-Hsp2Cas9

   Plasmid

   #192126

   Purpose

   Expresses Hsp2Cas9

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp2Cas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-Hsp1Cas9

   Plasmid

   #192125

   Purpose

   Expresses Hsp1Cas9

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1Cas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

   Plasmid

   #195504

   Purpose

   Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG, Puro, and T2A

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15543_dABE-SuperFi-Cas9

    Plasmid

    #184373

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-H2BC11_sgRNA

    Plasmid

    #183883

    Purpose

    pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-HypaCas9-MYH10_sgRNA

    Plasmid

    #183887

    Purpose

    pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-CfANLN_sgRNA

    Plasmid

    #183880

    Purpose

    pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN sgRNA spacer
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1137B=Hsp70Bb-Cas9

    Plasmid

    #153284

    Purpose

    The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330 C-FKBP-Cas9

    Plasmid

    #162729

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid

    #136140

    Purpose

    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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