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pSMP-MeCP2_1

Plasmid

#36377

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MeCP2 (MECP2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-shAUF1 hairpin 1

Plasmid

#52921

Purpose

expresses a hairpin specific for human AUF1

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting AUF1 (HNRNPD Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
pLKO-shAUF1 hairpin 2

Plasmid

#52922

Purpose

expresses a hairpin specific for human AUF1

Depositor

Sheila Stewart

Article

Alspach et al Cancer Discov. 2014 Mar 26.

Insert

shRNA targeting AUF1 (HNRNPD Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only
FnoCas12a-DR-cr in PBCSK-

Plasmid

#126642

Purpose

Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

Plasmid

#107323

Purpose

Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-NmCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-L207W-EFGP

Plasmid

#176900

Purpose

mouse ILK with L207W point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Leucine 207 to Tryptophane

Promoter

CNV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Scara3-IRES-BFP

Plasmid

#117265

Purpose

Retroviral overexpression of Scara3

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Scara3 (Scara3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

LTR

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-MDGA1

Plasmid

#82541

Purpose

Lentiviral vector for expressing HRP-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMX-Gal4-hMeCP2-R111G

Plasmid

#48085

Purpose

Mammalian expression of Gal4 tagged MeCP2-WT-R111G

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

Gal4

Expression

Mammalian

Mutation

R111G mutant MeCP2

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pSMP-MeCP2_3

Plasmid

#36379

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MeCP2 (MECP2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-uTEV1-MAVS-mTA

Plasmid

#170999

Purpose

HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[R537A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[R537A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSMP-DNMT1_3

Plasmid

#36384

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

DNMT1 (DNMT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-103)

Plasmid

#60357

Purpose

Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-103) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 7-103 of Msi1

Promoter

T7

Available since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only
B52 + TIMELESS sgSTOP

Plasmid

#100713

Purpose

B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting TIMELESS (cloned using BbsI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + PARP4 sgSTOP

Plasmid

#100711

Purpose

B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting PARP4 (cloned using BbsI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAM29 (wt)

Plasmid

#31143

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

WT

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pECE-HA-FOXO3 S574A

Plasmid

#52426

Purpose

mammalian expression of FOX03 with mutant JNK phosphorylation site

Depositor

Steven Weinman

Article

Tikhanovich et al Hepatology. 2014 Jan;59(1):58-70. doi: 1

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S574A, mutant JNK phosphorylation site

Promoter

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMX-Gal4-hMeCP2-G273X

Plasmid

#48084

Purpose

Mammalian expression of Gal4 tagged MeCP2-G273X

Depositor

Huda Zoghbi

Article

Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Tags

Gal4

Expression

Mammalian

Mutation

expresses AT-Hook G273X

Promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pSMP-Ehmt1_3

Plasmid

#36337

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only