We narrowed to 7,460 results for: Lif

1. Lenti-guide-puro mRab12-1

   Plasmid

   #198475

   Purpose

   lentiviral stable expression of mRab12 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mouse Rab12 (Rab12 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. siRNA-resistant ABHD16A-mNG

   Plasmid

   #195160

   Purpose

   mammalian expression of siRNA-resistant ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ABHD16A (ABHD16A Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from L90 to…

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Stim1NTD-ABHD16Acyto-mNG

   Plasmid

   #195158

   Purpose

   mammalian expression of ER localized ABHD16A tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   Stim1NTD (STIM1 Human)
   

   Use

   Tags

   ABHD16A cytoplasmic domain and mNeonGreen

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET303-ColE1-TR-E192C-del1-40

   Plasmid

   #180235

   Purpose

   Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   Colicin E1
   

   Use

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Truncation of ColE1 containing T and R domains (r…

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SNAP-Kindlin

   Plasmid

   #186136

   Purpose

   For expression of recombinant Snap-labeled kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CMV-Cyp26a1

   Plasmid

   #185563

   Purpose

   AAV construct to ubiquitously express mouse Cyp26a1

   Depositor

   Connie Cepko

   Article

   Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

   Insert

   Cyp26a1 (Cyp26a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUGW-scrambled

   Plasmid

   #183455

   Purpose

   This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   scrambled sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p8319 pDONR ATG-YAP1 WT-Stop

   Plasmid

   #184524

   Purpose

   Subcloning YAP1 WT

   Depositor

   Elizabeth White

   Article

   Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

   Insert

   YAP1 WT (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJET-10-mCherry-R11b

   Plasmid

   #179891

   Purpose

   Donor with homologous arms for Rab11b to knock in mCherry

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-BTLA (PD-1 ITIM)-mGFP

    Plasmid

    #180818

    Purpose

    Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

    Insert

    BTLA (PD-1 ITIM) (BTLA Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJH3453

    Plasmid

    #179577

    Purpose

    Pglc-55B::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVB/others premotor IN and others expression of miniSOG-SL2 BFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    BFP

    Expression

    Worm

    Mutation

    Promoter

    Plgc-55B

    Available since

    March 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJH2890

    Plasmid

    #179561

    Purpose

    Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Plgc-55B

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-eGFP-OPTN S473A 513A

    Plasmid

    #175763

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S473A S513A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Halo-WIPI2B H85E K88E

    Plasmid

    #175032

    Purpose

    Expresses WIPI2B (H85E K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

    Depositor

    Andrea Stavoe

    Article

    Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

    Insert

    WIPI2B (H85E K88E) (WIPI2 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

    Promoter

    CMV

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6.3delta4 GPR3-V5

    Plasmid

    #172601

    Purpose

    Lentiviral plasmid expressing c-terminal V5-tagged GPR3

    Depositor

    Robert Screaton

    Article

    Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

    Insert

    G protein-Coupled Receptor 3 (GPR3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMiniT:myc-GluA2 TKIT donor

    Plasmid

    #169423

    Purpose

    Donor DNA for TKIT knockin of Myc to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Myc (Myc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUE1-TSP-ORF68 K174A/R179A/K182A

    Plasmid

    #162654

    Purpose

    Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. UAS-u(XXL)Cas9

    Plasmid

    #127387

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XXL)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJET1.2_Nkx3-2_Anti-Sense

    Plasmid

    #124438

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJET1.2_Nkx3-2_Sense

    Plasmid

    #124437

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    NK3 homeobox 2 (Nkx3-2 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJET1.2_Rab28_Sense

    Plasmid

    #124441

    Purpose

    Plasmid for sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJET1.2_Rab28_Anti-Sense

    Plasmid

    #124442

    Purpose

    Plasmid for anti-sense in situ probe in vitro transcription

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Insert

    RAB28, member RAS oncogene family (Rab28 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

    Plasmid

    #114373

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv4.2LL (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv4.2LL

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

    Plasmid

    #114375

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Kv2.1C (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Kv2.1C

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

    Plasmid

    #114372

    Purpose

    expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-Nlgn1C (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and Nlgn1C

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Unc5c.a-AP-His

    Plasmid

    #72054

    Purpose

    Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.a (Unc5c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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