We narrowed to 4,048 results for: GAST
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Plasmid#113009PurposeFor bacterial expression of MBP fusion of N terminal region of Drosophila TralDepositorInsertN terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 1-355PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral FL-15A
Plasmid#113011PurposeFor bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)DepositorInsertfull length tral (tral Fly)
UseTagsExpressionBacterialMutation15 PNG phosphorylation sites mutated to APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C
Plasmid#113008PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila TralDepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationamino acids 356-652PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL c2x Tral C 15A
Plasmid#113007PurposeFor bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)DepositorInsertC terminus of tral (tral Fly)
UseTagsExpressionBacterialMutationPNG phosphorylation sites mutated to APromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4D03
Plasmid#70969PurposeGateway entry cloneDepositorInsertTaf12 (Taf12 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_CUS1F03
Plasmid#71071PurposeGateway entry cloneDepositorInsertSu(var)205 (Su(var)205 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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BII-CMV-AfmW3A
Plasmid#133402PurposePiggybac vector for constitutive expression of Afamin + Wnt3a for production of conditioned media in HEK293T cellsDepositorUseTagsExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-g-osk-TRICK
Plasmid#71244Purposeoskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.DepositorInsertoskar (osk Fly)
UseP element-mediated germline transformationTags12xPP7 and 6xMS2ExpressionInsectMutationPromoteroskAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA
Plasmid#211817PurposeAAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNADepositorInsertN-terminus of SpCas9-ABE8e and tracrRNA
UseAAVTagsExpressionMammalianMutationPromoterchicken β-actin promoter and U6 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA
Plasmid#211818PurposeAAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNADepositorInsertC-terminus of SpCas9-ABE8e and tracrRNA
UseAAVTagsExpressionMammalianMutationPromoterchicken β-actin promoter and U6 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-EGFP-Actin5c
Plasmid#15312DepositorInsertActin5c (Act5C Fly)
UseTagsEGFP and HisExpressionInsectMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-dnTCF4-ires-GFP
Plasmid#133378PurposePiggybac vector for constitutive expressionDepositorInsertdnTCF4 (TCF4 Human)
UseTags2x FlagExpressionMammalianMutationdeltaN31PromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-IMP2-2_antisense-control
Plasmid#42174DepositorInsertInsulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterT7Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pJC-elav1.8kb-TALE1-VP64-P10
Plasmid#104609PurposeExpresses TALE1 under the control of a 1.8kb elav enhancer elementDepositorInsert1.8kb elav enhancer element (elav Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Moe-pDONR221
Plasmid#124239PurposeGateway entry clone of Drosophila Moesin cDNA encoding the D isoform.DepositorInsertDrosophila Moesin (Moe Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4C03
Plasmid#70961PurposeGateway entry cloneDepositorInsertHis2Av (His2Av Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159677PurposePlasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.DepositorInsert[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N1 Btsz2
Plasmid#40068DepositorInsertBtsz2 (btsz Fly)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUAS-spatzle-HA
Plasmid#240235PurposeExpression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.DepositorInsertspatzle (spz Fly)
UseTagsHA-spGFP11ExpressionInsectMutationPromoterUASAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA
Plasmid#211819Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishmentDepositorInsertSpCas9, tracrRNA and blasticidin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter and miniU6 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro
Plasmid#211821PurposePiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishmentDepositorInsertSpCas9-ABE8e, tracrRNA and Puromycin resistance gene
UseTagsExpressionMammalianMutationPromoterchicken β-actin promoter, U6 promoter and hPGK pr…Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR
Plasmid#211815Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishmentDepositorInsertSpCas9, tracrRNA and puromycin selection gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter, U6 promoter, and hPGK promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD
Plasmid#148514PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83DepositorInsertDmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD
Plasmid#148515PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-675DepositorInsertDmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717_X
Plasmid#147982PurposeInsect Expression of DmNot1_1-1717DepositorInsertDmNot1_1-1717 (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_X
Plasmid#147986PurposeInsect Expression of DmNot1_1-1717-K1537SG1562EQ1658ADepositorInsertDmNot1_1-1717-K1537SG1562EQ1658A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_X
Plasmid#147987PurposeInsect Expression of DmNot1_1-1717-R1197AN1203AF1204ADepositorInsertDmNot1_1-1717-R1197AN1203AF1204A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_X
Plasmid#147988PurposeInsect Expression of DmNot1_1-1717-RNFKGQtoAAASEADepositorInsertDmNot1_1-1717-RNFKGQtoAAASEA (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1945-dsRNAres_X
Plasmid#147989PurposeInsect Expression of DmNot1_1-1945-dsRNAresDepositorInsertDmNot1_1-1945-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation8 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-G1562E-dsRNAres_X
Plasmid#147996PurposeInsect Expression of DmNot1-G1562E-dsRNAresDepositorInsertDmNot1-G1562E-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-K1537SG1562EQ1658A-dsRNAres_W
Plasmid#147876PurposeInsect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAresDepositorInsertDmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-K1537SQ1658A-dsRNAres_W
Plasmid#147877PurposeInsect Expression of DmNot1-K1537SQ1658A-dsRNAresDepositorInsertDmNot1-K1537SQ1658A-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-RNFtoAAA-dsRNAres_W
Plasmid#147878PurposeInsect Expression of DmNot1-RNFtoAAA-dsRNAresDepositorInsertDmNot1-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only