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pCSIIbleo-miRFP670nano-P2A-EGFP

Plasmid

#198064

Purpose

The lentiviral vector for miRFP670nano and EGFP

Depositor

Michiyuki Matsuda

Insert

miRFP670nano

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP720-T2A-HO1

Plasmid

#197248

Purpose

Expresses the protein of wmiRFP720-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP720-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-hygro-3Ty:tagRFPt:3Ty

Plasmid

#201088

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-3Ty:tagRFPt:3Ty

Plasmid

#201087

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3Ty:tagRFPt:3Ty

Plasmid

#201086

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wiRFP713-T2A-HO1

Plasmid

#197247

Purpose

Expresses the protein of wiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wiRFP713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-iRFP713-HO1

Plasmid

#197256

Purpose

Expresses the protein of iRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-emiRFP670-HO1

Plasmid

#197252

Purpose

Expresses the protein of emiRFP670 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP670

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Use

Tags

Expression

Worm

Mutation

Promoter

myo-2

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Borealin-mTAG-RFPt-3XFLAG

Plasmid

#191986

Purpose

Mammalian expression of PTK Borealin

Depositor

Paul Maddox

Insert

Borealin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP2-IRES-HO1-N1

Plasmid

#178967

Purpose

Expresses miRFP2 and HO1 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

miRFP2

Use

Tags

HO1 and no

Expression

Mammalian

Mutation

NO

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-miRFP670-eDHFR(69K6)

Plasmid

#178852

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-eDHFR(69K6)-iRFP713

Plasmid

#178855

Purpose

Mammalian expression of eDHFR(69K6)-iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

eDHFR(69K6)-iRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-miRFP670-Zdk1-MCS

Plasmid

#178873

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of miRFP670-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

miRFP670-Zdk1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH1-TRIO

Plasmid

#176119

Purpose

Heterodimerization with iLID, Rac1 and RhoG activation

Depositor

Dorus Gadella

Insert

TRIO (TRIO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.4.EFS-NS.H2B-RFP

Plasmid

#170365

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS-NS

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.3.EFS-NS.H2B-RFP

Plasmid

#170364

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EFS-NS

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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ystagRFP-T2A-mTq2 pdrf1-GW

Plasmid

#168072

Purpose

Produces equimolar expression of ystagRFP and mCherry for use in yeast

Depositor

Bas Teusink

Insert

ystagRFP-T2A-mTq2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1

Plasmid

#160448

Purpose

BioBrick mammalian-codon-optimized mRFP1

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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