-
Plasmid#121994PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-opa1 guide
Plasmid#121995PurposeAn entry vector with U6a and U6c promoter driving opa1 guide RNAs expressionDepositorInsertopa1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265H
Plasmid#149554PurposeGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMsDepositorInserthAID-ziSpyMacCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266B
Plasmid#149555PurposeGateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMsDepositorInsertziSpyMacCas9(D10A)-PmCDA1-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Centrin3
Plasmid#136855PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin3
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Centrin3
Plasmid#136856PurposeMammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tagDepositorInsertCLIP-Centrin3
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-FOP
Plasmid#136846PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tagDepositorInsertCLIP-FGFR
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-POC5
Plasmid#136836PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tagDepositorInsertCLIP-POC5 (POC5 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-FOP
Plasmid#136845PurposeMammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tagDepositorInsertCLIP-FGFR
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-POC5
Plasmid#136835PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tagDepositorInsertCLIP-POC5 (POC5 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-POC5
Plasmid#136787PurposeMammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tagDepositorInsertSNAP-POC5 (POC5 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
JK305: pMVP/Blast-DEST
Plasmid#121855PurposepMVP destination vector, empty expression vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX-eSOX2NRR
Plasmid#118825PurposeRetroviral expression of a mutant of Sox2 that improves efficiency of iPSC generationDepositorInserteSOX2NRR (Sox2 Mouse)
UseRetroviralTagsExpressionMammalianMutationNRR: E46N, I53R, K57RPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-Citrine-SV40pA-FKF
Plasmid#119865PurposeDonor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R20
Plasmid#101819PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G14-R20
Plasmid#101821PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R11
Plasmid#101809PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G11-R20
Plasmid#101820PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G20-R18
Plasmid#101817PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G14-R18
Plasmid#101816PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G11-R18
Plasmid#101815PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R18
Plasmid#101814PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G20-R11
Plasmid#101812PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G14-R11
Plasmid#101811PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G11-R11
Plasmid#101810PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v2
Plasmid#79538PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 2DepositorInsertAmrose v2
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Alx3
Plasmid#80382Purposemammalian expression of rat Alx3DepositorInsertAlx3 (Alx3 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 G108C/P186C
Plasmid#66174PurposeExpresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108C, P186CPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGS1422
Plasmid#63793PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection markerDepositorInserteCitrine-Twin-tag followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Twin-tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 EE Jip1b
Plasmid#52127Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)
UseTagsEEExpressionMammalianMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only