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pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

Plasmid

#169498

Purpose

Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven YPet-P2aA-PuroR

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 632-639D

Plasmid

#175771

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

632-639D

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN D474N

Plasmid

#175762

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Mutation

D474N

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCm N-term MBP SMT3 NikJC199U

Plasmid

#174363

Purpose

Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis, MBP, SMT3, and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Tpi1

Plasmid

#175940

Purpose

knockout mouse Tpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Tpi1 gRNA (Tpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Olfr2

Plasmid

#175935

Purpose

knockout mouse Olfr2

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Olfr2 gRNA (Or6a2 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid1

Plasmid

#173469

Purpose

N-terminal region of PGRMC2 fused to the transmembrane and C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid2

Plasmid

#173470

Purpose

N-terminal and transmembrane region of PGRMC2 fused to the C-terminal region of PGRMC1

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC-Hybrid3

Plasmid

#173471

Purpose

N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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4LNDD-pCDNA3.1(+)

Plasmid

#172227

Purpose

Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

Lambda N peptide - human ADAR2 Deaminase Domain

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Plasmid

#159794

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAmreB

Plasmid

#149595

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAftsI

Plasmid

#149591

Purpose

all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Expression

Bacterial

Promoter

PflaB, PpQE30(-10TC), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide#2

Plasmid

#121994

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-opa1 guide

Plasmid

#121995

Purpose

An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

opa1 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ265H

Plasmid

#149554

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

hAID-ziSpyMacCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ266B

Plasmid

#149555

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ziSpyMacCas9(D10A)-PmCDA1-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Centrin3

Plasmid

#136855

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Centrin3

Plasmid

#136856

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-FOP

Plasmid

#136846

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-POC5

Plasmid

#136836

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-POC5 (POC5 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-FOP

Plasmid

#136845

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-POC5

Plasmid

#136835

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-POC5 (POC5 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-POC5

Plasmid

#136787

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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JK305: pMVP/Blast-DEST

Plasmid

#121855

Purpose

pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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