We narrowed to 17,375 results for: puro

1. PL-SIN-EOS-C(3+)-EiP

   Plasmid

   #21313

   Purpose

   Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EOS-C(3+) promoter region

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJR103

   Plasmid

   #187242

   Purpose

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXL002-ishRNA-beta-catenin-1

   Plasmid

   #36297

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

4. PL-SIN-EOS-S(4+)-EiP

   Plasmid

   #21314

   Purpose

   Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EOS-S(4+) promoter region

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pY30

   Plasmid

   #84745

   Purpose

   Expresses huAsCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXL003-ishRNA-beta-catenin-2

   Plasmid

   #36299

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of beta-catenin-2 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pAAVS1-PdTK-CAG-mCh-[uBglII]

   Plasmid

   #107280

   Purpose

   puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. crTet in pSLQ8453

   Plasmid

   #183965

   Purpose

   U6-crTet-EF1a-Puro-T2A-BFP-WPRE

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   crTet
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SaLgCP

    Plasmid

    #164562

    Purpose

    Puro

    Depositor

    Junwei Shi

    Article

    Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Flag-NLS-SaCas9-P2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-PIG-FLAG-TAF4(827-909)

    Plasmid

    #105617

    Purpose

    retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPB9P2

    Plasmid

    #210030

    Purpose

    Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPB9P3

    Plasmid

    #210034

    Purpose

    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-Creb5(HA)

    Plasmid

    #195024

    Purpose

    HA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV CMV FST-HA

    Plasmid

    #175168

    Purpose

    Lentiviral expression of human FST-HA

    Depositor

    Larry Gerace

    Insert

    FST
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO-sh-BNIP3-FL

    Plasmid

    #100771

    Purpose

    Lentiviral expression of shRNA targeting BNIP3

    Depositor

    Joseph Gordon

    Insert

    Lenti-sh-BNIP3-FL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRosetta

    Plasmid

    #59700

    Purpose

    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

    Depositor

    David Root, John Doench

    Inserts

    Puro
    BlastR
    EGFP
    

    Use

    Lentiviral

    Tags

    2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiGuide-BlastR

    Plasmid

    #196970

    Purpose

    Lentiviral acceptor vector for pegRNAs with blasticidin resistance

    Depositor

    Leopold Parts

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGGSG-FKBP-2HA-P2a-Pac

    Plasmid

    #235521

    Purpose

    C-term tagging construct: clone into PmlI and AfeI sites

    Depositor

    Brian Hendrich

    Insert

    FKBP
    

    Use

    Mouse Targeting

    Tags

    FKBP, 2xHA, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CROP-seq-opti-dsRed

    Plasmid

    #201999

    Purpose

    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

    Depositor

    Daniel Geschwind

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGGSG-Bromo-T7-T2a-Pac

    Plasmid

    #235518

    Purpose

    Cloning vector for GGSG-BromoTag-T7-T2A-Pac. Clone into PmlI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGGSG-Bromo-T7-P2a-Pac

    Plasmid

    #235517

    Purpose

    Cloning vector for GGSG-BromoTag-T7-P2A-Pac. Clone into PmlI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-EF1a-V5-Tmem128

    Plasmid

    #120239

    Purpose

    Expresses V5-tagged Tmem128 in lentiviral vector

    Depositor

    Larry Gerace

    Insert

    Tmem128 (Tmem128 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV Rnf121-V5

    Plasmid

    #175130

    Purpose

    Lentiviral expression of mouse Rnf121-V5

    Depositor

    Larry Gerace

    Insert

    Rnf121 (Rnf121 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mem-mCherry-pTRIP

    Plasmid

    #163521

    Purpose

    Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    membrane targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV Nup62-V5

    Plasmid

    #175116

    Purpose

    Lentiviral expression of mouse Nup62-V5

    Depositor

    Larry Gerace

    Insert

    Nup62 (Nup62 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. nuc-mCherry-pTRIP

    Plasmid

    #163520

    Purpose

    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    nucleus targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti_PGK_Prkaa1

    Plasmid

    #204356

    Purpose

    Lentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoter

    Depositor

    Günter Schneider

    Insert

    AMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV Tor1a-V5

    Plasmid

    #175135

    Purpose

    Lentiviral expression of Tor1a-Myc

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV V5-Mospd1

    Plasmid

    #175132

    Purpose

    Lentiviral expression of V5-tagged mouse Mospd1

    Depositor

    Larry Gerace

    Insert

    Mospd1 (Mospd1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV Tyw1-V5

    Plasmid

    #175131

    Purpose

    Lentiviral expression of mouse Tyw1-V5

    Depositor

    Larry Gerace

    Insert

    Tyw1 (Tyw1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. StarShip01

    Plasmid

    #164505

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. StarShip02

    Plasmid

    #164506

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. StarShip03

    Plasmid

    #164507

    Purpose

    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.

    Depositor

    Bo Huang

    Type

    Empty backbone

    Use

    Bxbi site specific integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CROP-seq-copGFP-v2-no-scaffold

    Plasmid

    #228520

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MSCV-PIG-N3FLAG-TAF12(50-130)

    Plasmid

    #105595

    Purpose

    retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    truncation fragments containing aa (50-130), and …

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLIX_403

    Plasmid

    #41395

    Purpose

    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLV V5-Nesprin-3

    Plasmid

    #175136

    Purpose

    Lentiviral expression of V5-tagged mouse Syne3

    Depositor

    Larry Gerace

    Insert

    Syne3 (Syne3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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