We narrowed to 4,320 results for: AR

1. pDONR223-TAF5-FL

   Plasmid

   #209047

   Purpose

   Gateway-compatible entry vector, with insert of the full length TAF5 gene

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 full length
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

   Plasmid

   #154910

   Purpose

   1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

   Depositor

   Clare Buckley, Jonathan Clarke

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed, Synthetic)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-IRES-blast GFP-SPNS1

   Plasmid

   #198571

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. NKP90 RFP (787)

   Plasmid

   #62001

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   NKP90 (TMEM126A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   pgk

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pScarlessHD-C-3xVHH05-DsRed

   Plasmid

   #171580

   Purpose

   For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3xVHH05 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

   Plasmid

   #216763

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

   Plasmid

   #113436

   Purpose

   Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES2-DsRed-MST-Kv2.1

   Plasmid

   #131707

   Purpose

   Expresses Kv2.1in mammalian cells

   Depositor

   Jennifer Kearney

   Article

   Jorge et al Proc Natl Acad Sci U S A. 2011 Mar 29;10

   Insert

   KCNB1 (KCNB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

   Plasmid

   #194464

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-JAG1 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Myh6-Cas9

    Plasmid

    #109037

    Purpose

    Cardiac-specific overexpression of Cas9

    Depositor

    Eva van Rooij

    Article

    Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

    Insert

    Cas9
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    alpha MHC

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKOv3

    Plasmid

    #209025

    Purpose

    Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScarlessHD-C-3x127D01-DsRed

    Plasmid

    #171578

    Purpose

    For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCsy_complex

    Plasmid

    #89232

    Purpose

    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Inserts

    Csy1
    Csy2
    Csy3
    Csy4
    

    Use

    Tags

    6xHis-TEV protease

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+-Y276H PHYB-CAAX

    Plasmid

    #154911

    Purpose

    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

    Depositor

    Clare Buckley, Jonathan Clarke

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR

    Plasmid

    #136514

    Purpose

    Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector

    Depositor

    Erik Sontheimer

    Article

    Garcia et al Cell Rep. 2019 Nov 12;29(7):1739-1746.e5

    Insert

    Codon-optimized AcrIIA5
    

    Use

    Lentiviral

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScarlessHD-N-3xVHH05-DsRed

    Plasmid

    #171581

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 TREK-2

    Plasmid

    #224769

    Purpose

    In vitro transcription for Oocyte expression of 1 TREK-2

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 10 (Kcnk10 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-IV-GFPL10

    Plasmid

    #98704

    Purpose

    Cre-dependently express GFPL10 fusion using Introvert Switch

    Depositor

    Jeffrey Friedman, Alexander Nectow

    Article

    Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

    Insert

    GFPL10
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L10: 2609 C->T to remove EcoRI site

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

    Plasmid

    #166804

    Purpose

    Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S1213A

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 6A CARD8 CARD-FLAG WT

    Plasmid

    #164020

    Purpose

    Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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