We narrowed to 733 results for: Tcta

1. pPN145

   Plasmid

   #91674

   Purpose

   Express sgRNA targeting human TBC1D5

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   TBC1D5 (TBC1D5 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-BbsI-Btl

   Plasmid

   #244934

   Purpose

   CRISPR gRNA targeting the C-terminal end of Breathless for cleavage.

   Depositor

   Jared Toettcher

   Article

   Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

   Insert

   gRNA targeting Btl C terminus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX459_L31_CRISPR_upstream_sgRNA

   Plasmid

   #235609

   Purpose

   Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   Rpl31 C-terminus upstream sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. inducible shSRP19_9 in pLKO tet on

   Plasmid

   #233289

   Purpose

   Dox inducible expression of SRP19 shRNA

   Depositor

   Sefi Rosenbluh

   Article

   Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

   Insert

   shSRP19
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dox

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210734

   Purpose

   Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Frodo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MS2-adRNA (RAB7A-GAC site)

   Plasmid

   #170140

   Purpose

   AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-RAB7A(GAC)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCF821-sgOR2B6-1_U6-sgRNA-EF1a-mNeonGreen

   Plasmid

   #211655

   Purpose

   U6-sgRNA-EF1a-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and sgOR2B6-1 guide RNA vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCF821-sgRPA1-3_U6-sgRNA-EF1a-mNeonGreen

   Plasmid

   #211660

   Purpose

   U6-sgRNA-EF1a-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and sgRPA1-3 guide RNA vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCF821-sgCIDE-23_U6-sgRNA-EF1a-mNeonGreen

   Plasmid

   #211673

   Purpose

   U6-sgRNA-EF1a-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and sgCIDE-23 guide RNA vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210736

    Purpose

    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Merry sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HSF2 KO

    Plasmid

    #200208

    Purpose

    gRNA for HSF2 KO

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    HSF2 KO (Hsf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB1839

    Plasmid

    #193103

    Purpose

    GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

    Insert

    GB_SynP gRNA2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCL.107

    Plasmid

    #184976

    Purpose

    Test effect of extending a1/a2 on CAN1 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CAN1 donor G444stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL.106

    Plasmid

    #184975

    Purpose

    Express -Eco1 CAN1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CAN1 donor G444stop

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc-sgRNA-Cas9.dora_3

    Plasmid

    #190702

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 3
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pA-RFP-rG2

    Plasmid

    #188969

    Purpose

    IPTG inducible mCherry with sgRNA

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    mCherry
    sgRNA: agtccatgtaatcagcgtctactagt
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458_miR-290-295KO-gRNA3

    Plasmid

    #172711

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1056K

    Plasmid

    #183137

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK17 pCAS-Tyr-[gRNA: 7=HIS3] (SplitKanR, AmpR)

    Plasmid

    #179001

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Eomes gRNA#1

    Plasmid

    #170834

    Purpose

    Cas9-mediated knockout of Eomes in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Eomes (Eomes Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBbdCas9S(-10AC1)_Psyn-sgRNArodA

    Plasmid

    #149659

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC1), Psyn

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBbdCas9S(-10AC2)_Psyn-sgRNArodA

    Plasmid

    #149660

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC2), Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBbdCas9S(-10TC)_Psyn-sgRNArodA

    Plasmid

    #149657

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAPM-D4 miR30-SUV39h1 ts3

    Plasmid

    #115882

    Purpose

    SUV39h1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h1 shRNA (SUV39H1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid

    #178091

    Purpose

    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BRCA1 A10.2 gRNA

    Plasmid

    #90549

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BRCA1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BRCA1 (Guide Designation A10.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

    Plasmid

    #138988

    Purpose

    IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. sgPBRM1-1- LentiCRISPRv2

    Plasmid

    #107403

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiCRISPRpuro-sgROSA26

    Plasmid

    #231562

    Purpose

    pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

    Depositor

    Rizwan Haq

    Article

    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

    Insert

    sgROSA26
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF-1a

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

    Plasmid

    #122334

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLL5.0 shFmnl2-EGFP

    Plasmid

    #187255

    Purpose

    Lentiviral expression of shRNA targeting Fmnl2

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Fmnl2 (Fmnl2 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Circular 100,50 (RAB7A)

    Plasmid

    #170117

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 100,50 guide RNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Circular 200,100 with interspersed loops (SMAD4)

    Plasmid

    #180191

    Purpose

    AAV vector carrying a guide RNA targeting the human SMAD4 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. E42_gRunx1_gRNA1_dTet_NGFR

    Plasmid

    #189802

    Purpose

    Retroviral delivery of guide RNA against mouse Runx1

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx1_gRNA1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PX458_KDM1A_1

    Plasmid

    #86297

    Purpose

    Encodes gRNA for 3' target of human KDM1A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM1A (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLKO-puro-tetON-shGFP

    Plasmid

    #110939

    Purpose

    Tet-inducible shRNA targeting GFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PX458_KDM1A_2

    Plasmid

    #86298

    Purpose

    Encodes gRNA for 3' target of human KDM1A

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM1A (KDM1A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge