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Showing: 351 - 400 of 4186 results

  16. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Article
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Use
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  24. pET20b_Pa-PirA

    Plasmid
    #128956
    Purpose
    expresses Pseudomonas aeruginosa PirA in E. coli
    Depositor
    James Naismith
    Article
    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24
    Insert
    ferric enterobactin receptor PirA (pirA Pseudomonas aeruginosa PAO1, Synthetic)
    Use
    Tags
    6xHis and pelB leader
    Expression
    Bacterial
    Mutation
    Signal peptide sequence has been removed
    Promoter
    T7
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128956-plasmid-map-sequence-id-254679

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  26. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  31. pET21a IHF

    Plasmid
    #26641
    Depositor
    Phoebe Rice
    Insert
    IHF
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 22, 2010
    Availability
    Academic Institutions and Nonprofits only

  34. pET28GST-LIC

    Plasmid
    #26101
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6XHis, GST, and Thrombin CLeavage Site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26101-plasmid-map-sequence-id-142370

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  38. pET28a-B4E

    Plasmid
    #162067
    Purpose
    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E
    Depositor
    Karen Polizzi
    Article
    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1
    Insert
    ampR-eIF4E fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    eIF4E incorporating the K119A mutation, which is …
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162067-plasmid-map-sequence-id-325536

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  47. pET22b-EC12WTC9AAvi

    Plasmid
    #47925
    Purpose
    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.
    Depositor
    Tanja Kortemme
    Article
    Ritterson et al J Am Chem Soc. 2013 Aug 7.
    Insert
    E-Cadherin EC12
    Use
    Tags
    6xHis, Avi Tag, and TEV Cleavage
    Expression
    Bacterial
    Mutation
    changed Cysteine 9 to Alanine
    Promoter
    T7
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47925-plasmid-map-sequence-id-68712

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  50. pET21-Drp1

    Plasmid
    #72927
    Purpose
    Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site
    Depositor
    David Chan
    Article
    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77
    Insert
    Drp1 (Dnm1l Mouse)
    Use
    Tags
    6xHis-tag and PreScission Protease site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72927-plasmid-map-sequence-id-137706

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Showing: 351 - 400 of 4186 results