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Showing: 351 - 400 of 638 results

  4. pZH051

    Plasmid
    #47311
    Purpose
    Expresses Tsr-Venus-Ubiquitin-CI
    Depositor
    Jie Xiao
    Article
    Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-
    Insert
    wild type immunity region of lambda phage with tsr-venus-ub-cI
    Use
    Tags
    CI
    Expression
    Bacterial
    Mutation
    Promoter
    PR; PRM
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pGDP3 arr

    Plasmid
    #112890
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    arr
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112890-plasmid-map-sequence-id-219900

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  8. pGDP1 fosA

    Plasmid
    #112889
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    fosA
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112889-plasmid-map-sequence-id-219899

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  12. pAGH51

    Plasmid
    #107255
    Purpose
    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    HU-FRB
    Use
    Synthetic Biology
    Tags
    FRB
    Expression
    Mutation
    Promoter
    native HU promoter
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107255-plasmid-map-sequence-id-207562

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  18. dSp-Rta Full (1-190)

    Plasmid
    #99662
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain (1-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99662-plasmid-map-sequence-id-192021

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  19. pYG205

    Plasmid
    #200836
    Purpose
    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P-BAD
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200836-plasmid-map-sequence-id-404931

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  20. pRnc2-gGFP

    Plasmid
    #189563
    Purpose
    Expresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFP
    Depositor
    Marc Ostermeier
    Article
    Weeks et al Mol Biol Evol. 2023 Feb 27:msad047. doi:
    Inserts
    RNase III
    gUTR-129-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Maintains 5' UTR of rnc
    Promoter
    J23119 and pBAD
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    189563-plasmid-map-sequence-id-372779

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  23. pJH858

    Plasmid
    #172176
    Purpose
    PylB overexpression and purification
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172176-plasmid-map-sequence-id-339055

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  24. pJH859

    Plasmid
    #172177
    Purpose
    PylB overexpression and purification
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172177-plasmid-map-sequence-id-338578

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  31. pDM_N1_400

    Plasmid
    #170474
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Article
    Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282
    Insert
    T7A1, O-, O1, Lambda t1 in modified lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    diminished hairpins near O1 and diminished latent…
    Promoter
    T7A1
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170474-plasmid-map-sequence-id-336814

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  36. pGDP1 uvrA

    Plasmid
    #112892
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    uvrA-like protein
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112892-plasmid-map-sequence-id-219901

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  37. pGDP1 stat

    Plasmid
    #112886
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    stat
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112886-plasmid-map-sequence-id-219860

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  40. pEND-349_Cacnes_ori

    Plasmid
    #225604
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225604-plasmid-map-sequence-id-451376

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  41. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

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  48. AY27_pU6.gRNA.CLYBL

    Plasmid
    #199238
    Purpose
    Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    S. pyogenes gRNA spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199238-plasmid-map-sequence-id-391387

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  49. AD59_pEP.DonorCLYBL.TS

    Plasmid
    #199227
    Purpose
    Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Insert
    Expression unit for PuroR.T2A.EGFP selectable and reporter markers
    Use
    Gene targeting donor plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human PGK1 gene promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199227-plasmid-map-sequence-id-391392

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Showing: 351 - 400 of 638 results