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We narrowed to 653 results for: pBR322

Showing: 351 - 400 of 653 results

  1. pMP11

    Plasmid
    #215559
    Purpose
    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin
    Depositor
    Brian Pfleger
    Article
    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10
    Inserts
    Cas9
    gRNA targeting pBR322 origin of replication
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23105 and tet
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    215559-plasmid-map-sequence-id-434132

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  2. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid
    #228360
    Purpose
    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36
    Depositor
    Michael Boettcher
    Article
    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Rfxcas13d grna expression …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    228360-plasmid-map-sequence-id-454335

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  3. p5155

    Plasmid
    #175391
    Purpose
    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Michael Wiles
    Article
    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    175391-plasmid-map-sequence-id-348372

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  13. pET30b_T7Sav

    Plasmid
    #138588
    Purpose
    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    T7-tagged streptavidin variant with native C-terminus
    Use
    Synthetic Biology
    Tags
    T7-tag
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138588-plasmid-map-sequence-id-274485

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  15. pAL3

    Plasmid
    #114673
    Purpose
    T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.
    Depositor
    William Studier
    Article
    Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6
    Type
    Empty backbone
    Use
    Tags
    optional Ser-Gly-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    114673-plasmid-map-sequence-id-254326

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  17. PBDGTV

    Plasmid
    #100859
    Purpose
    A piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.
    Depositor
    Yue Huang
    Article
    Liu et al Nucleic Acids Res. 2017 Sep 28. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    100859-plasmid-map-sequence-id-198848

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  21. pZH051

    Plasmid
    #47311
    Purpose
    Expresses Tsr-Venus-Ubiquitin-CI
    Depositor
    Jie Xiao
    Article
    Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-
    Insert
    wild type immunity region of lambda phage with tsr-venus-ub-cI
    Use
    Tags
    CI
    Expression
    Bacterial
    Mutation
    Promoter
    PR; PRM
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pAGH51

    Plasmid
    #107255
    Purpose
    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    HU-FRB
    Use
    Synthetic Biology
    Tags
    FRB
    Expression
    Mutation
    Promoter
    native HU promoter
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107255-plasmid-map-sequence-id-207562

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  31. dSp-Rta Full (1-190)

    Plasmid
    #99662
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    Tags
    truncated RTA activation domain (1-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99662-plasmid-map-sequence-id-192021

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  32. pYG205

    Plasmid
    #200836
    Purpose
    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P-BAD
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200836-plasmid-map-sequence-id-404931

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  33. pRnc2-gGFP

    Plasmid
    #189563
    Purpose
    Expresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFP
    Depositor
    Marc Ostermeier
    Article
    Weeks et al Mol Biol Evol. 2023 Feb 27:msad047. doi:
    Inserts
    RNase III
    gUTR-129-GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Maintains 5' UTR of rnc
    Promoter
    J23119 and pBAD
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    189563-plasmid-map-sequence-id-372779

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  37. pJH859

    Plasmid
    #172177
    Purpose
    PylB overexpression and purification
    Depositor
    Matthew Bennett
    Article
    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:
    Insert
    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172177-plasmid-map-sequence-id-338578

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  44. pDM_N1_400

    Plasmid
    #170474
    Purpose
    To amplify transcription template containing LacI operators
    Depositor
    Laura Finzi
    Article
    Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282
    Insert
    T7A1, O-, O1, Lambda t1 in modified lacZ fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    diminished hairpins near O1 and diminished latent…
    Promoter
    T7A1
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170474-plasmid-map-sequence-id-336814

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Showing: 351 - 400 of 653 results