We narrowed to 6,346 results for: rel
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Plasmid#107466PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation T439M, decreased surface expression and transport relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationT439MPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG384S-myc
Plasmid#107463PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G384S with loss of surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG384SPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS375L-myc
Plasmid#107462PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S375L with lower surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS375LPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 990
Plasmid#52992PurposepLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
RhyB sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationincludes −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1022
Plasmid#52996PurposepLtetO-1:RhyB, pCon:OxyS, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
OxyS sRNA
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1020
Plasmid#52995PurposepLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA (competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1019
Plasmid#52994PurposepLtetO-1:RhyB, pCon:MicC, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
MicC sRNA (Competitor)
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1039
Plasmid#53000PurposepLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
OxyS sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1394
Plasmid#53009PurposepLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
T7(st3) (133bps)
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CW3SL-EGFP
Plasmid#61463PurposeAAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)DepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFR-PATagRFP
Plasmid#31950DepositorInsertPATagRFP
UseTagsEGF receptor (EGFR)ExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA11-mRuby3
Plasmid#184894PurposeStable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cellsDepositorInsertANXA11 (ANXA11 Human)
UseTagsmRuby3ExpressionMammalianMutationR230C relative to NP_001148.1PromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVenus-VSVG
Plasmid#11914DepositorInsertVSVG
UseTagsExpressionMammalianMutationF46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC57 APEX
Plasmid#40306PurposeA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).DepositorInsertpea APEX (Synthetic)
UseUnspecifiedTagsExpressionMutationK14D, W41F, E112K (relative to wild-type)PromoterAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
V246 pCEP 4HA B56beta
Plasmid#14533DepositorInsertPPP2R5B B56 beta regulatory subunit of PP2A (PPP2R5B Human)
UseTags4 HAExpressionMammalianMutationI346V relative to GenBank ID L42374PromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidTagsExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…PromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Medium-FT
Plasmid#31917DepositorInsertMedium-FT
UseTagsHisExpressionBacterialMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry-L
Plasmid#104002PurposePlasmid encoding mCherry-LDepositorInsertmCherry-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE DNLA IIKEF… V…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_W267N
Plasmid#214311PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationW267NPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST47-CypB-Del3 C-term GFP
Plasmid#36136DepositorInsertcyclophilin B (PPIB Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 39-216 of cyclophilin B; G63…PromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA
Plasmid#222404Purposearabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.DepositorInsert5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
UseTags1xMS2 hairpin, 7GC clamp, TtrpA terminatorExpressionBacterialMutationPromoterpBADAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_R269D
Plasmid#214310PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationR269DPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_K356D
Plasmid#214309PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationK356DPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K
Plasmid#183357PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 with ICE activity (CASP4 Human)
UseRetroviralTagsMycExpressionMutationANR261ENP, D315H, M318K, T352KPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0061
Plasmid#177026PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertiridoid oxidase; CYP76A26
UseSynthetic BiologyTagsExpressionMutationamplified from cDNA, contains T359S relative to K…PromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL1529
Plasmid#53027PurposePLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st2), RBS(st3), and glgC leader contains -61 …ExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEF… V…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Vimentin - APEX2 in pECFP
Plasmid#66170PurposeAPEX2 fused to the C-terminus human vimentinDepositorInserthuman Vimentin - APEX2 (VIM Human, Synthetic)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet
Plasmid#104006PurposePlasmid encoding mScarletDepositorInsertmScarlet
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221075PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCerulean-VSVG
Plasmid#11913DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
UseTagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRuby3 Addgene
Plasmid#104005PurposePlasmid encoding mRuby3 AddgeneDepositorInsertmRuby3 Addgene
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP611 (MSKGEE LIKENMRMKVV…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-941
Plasmid#103757PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-941 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRuby3
Plasmid#104004PurposePlasmid encoding mRuby3DepositorInsertmRuby3
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP611 (MSKGEE LIKENMRMKVV…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKG139
Plasmid#69755PurposeRetroviral expression of CENP-CDepositorInsertCENP-C (CENPC Human)
UseRetroviralTagsmCherry-TEV-SExpressionMammalianMutationL341F, N570D relative to NP_001803.2 (numbering b…PromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HseIF4AI_AC
Plasmid#148438PurposeMammalian Expression of HseIF4AIDepositorInsertHseIF4AI (EIF4A1 Human)
UseTagsExpressionMammalianMutation3 silent mutations relative to NM_001416.2PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D1D2
Plasmid#61492Purposeluciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf226
Plasmid#12914PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf226
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D1
Plasmid#61490Purposeluciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D2
Plasmid#61491Purposeluciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf67
Plasmid#12755PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf67
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-V5His6_H
Plasmid#146499PurposeInsect Expression of DmGW182DepositorInsertDmGW182 (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only