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FU-tet-o-hc-myc

Plasmid

#19775

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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HA-Akt DN (K179M)

Plasmid

#16243

Depositor

Mien-Chie Hung

Publication

Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-3

Insert

Akt1 (Akt1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (K179M)

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Publication

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Publication

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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EGFP-Bak

Plasmid

#32564

Depositor

Richard Youle

Publication

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Publication

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles
p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Publication

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSG5-MAGE-A11

Plasmid

#89096

Purpose

mammalian expression of full-length human MAGE-A11

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Expression

Mammalian

Promoter

SV40

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
CD44S pBabe-puro

Plasmid

#19127

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Expression

Mammalian

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Publication

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMSP1E2

Plasmid

#20063

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E2 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Publication

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Publication

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pdCas9-DNMT3A-EGFP

Plasmid

#71666

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pRPR1_gRNA_handle_RPR1t

Plasmid

#49014

Purpose

gRNA expression empty vector (yeast)

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

pRPR1

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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