We narrowed to 10,367 results for: ESP

1. pTSB-FICAT_Payload-Esp3I

   Plasmid

   #179380

   Purpose

   Transposon vector prepared for Esp3I golden gate cloning of Transgene

   Depositor

   Marc Güell

   Article

   Pallares-Masmitja et al Nat Commun. 2021 Dec 3;12(1):7071. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSARN-vasa-eSpCas9sv40

   Plasmid

   #173670

   Purpose

   eSpCas9 expression vector for Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Insert

   eSpCas9 with Anopheles vasa promoter and SV40 terminator
   

   Use

   CRISPR

   Expression

   Insect

   Mutation

   mutations in Cas9 reducing off-target activity (S…

   Promoter

   vasa

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)-Cdt1

   Plasmid

   #122342

   Purpose

   Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Cdt1
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BII-ChPtW-eSpCas9

   Plasmid

   #133381

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ESPL1 D7.1 gRNA

   Plasmid

   #90682

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ESPL1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ESPL1 (Guide Designation D7.1)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTagBFP2-Espin-Like-C-18

   Plasmid

   #62784

   Purpose

   Localization: Actin Bundling , C1 Cloning Vector, Excitation:399, Emission:456

   Depositor

   Michael Davidson

   Insert

   EspinLike
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. mEmerald-Espin-like-N-18

   Plasmid

   #54087

   Purpose

   Localization: Actin Bundling, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Espin-like
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Ki67∆FHA-mNeonGreen-IRESpuro2

   Plasmid

   #183739

   Purpose

   Expresses Ki67∆FHA-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67_lacking FHA (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking FHA)

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-eSpCas9-NLS-6xHis

    Plasmid

    #207379

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF1-alpha

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. V51 pIRESpuro-GLUE (pGLUE)

    Plasmid

    #15100

    Purpose

    tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Type

    Empty backbone

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMC-L1-Reverse-Donor-Esp3I

    Plasmid

    #197845

    Purpose

    Donor vector to restore L1 destination via Esp3I (reverse orientation)

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    None
    

    Use

    Unspecified

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. M77 pIRESpuro-GLUE-hDsh3

    Plasmid

    #15102

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    Dsh3 (DVL3 Human)
    

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. M76 pIRESpuro-GLUE-hDsh2

    Plasmid

    #15101

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    Dsh2 (DVL2 Human)
    

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ki67∆Nterm-mNeonGreen-IRESpuro2

    Plasmid

    #183740

    Purpose

    Expresses Ki67∆Nterm-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + all Ki-67 repeats (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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