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AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

Plasmid

#154003

Purpose

Vector expressed CasRx and sgRNA_LACZ for AAV package

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Tags

Expression

Mutation

Promoter

EFS

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-dSa-VPR mini.

Plasmid

#99686

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a-short; U6

Available since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pHAGE-EFS-PCP-3XNeon-nls

Plasmid

#75386

Purpose

PCP-3XNeon-nls

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XmNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

Plasmid

#192148

Purpose

T cell CRISPR AAV vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-foldon-GFP- PCP

Plasmid

#194503

Purpose

Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Insert

foldon-GFP-PCP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α core promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-EFS-HypaSpCas9-P2A-EGFP

Plasmid

#106964

Purpose

single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

Depositor

Alex Hewitt

Insert

HypaSpCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pOT_1 - lenti-EFS-LTBR-2A-puro

Plasmid

#181970

Purpose

Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

LTBR (LTBR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLY099-RetroEFS-BCMABBz-PRODH2-WPRE

Plasmid

#192200

Purpose

Retro-BCMACAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-BCMACAR-PRODH2 (TNFRSF17 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRC10-EFS-huLbCpf1-2A-Blast-WPRE

Plasmid

#123359

Purpose

Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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CROPseq-EFS-SpCas9-P2A-blast

Plasmid

#106477

Purpose

single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

Depositor

Alex Hewitt

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLY097-RetroEFS-CD22BBz-PRODH2-WPRE

Plasmid

#192198

Purpose

Retro-CD22CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR-PRODH2 (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEFS-ccdB-12X MS2_mPGK-Puro

Plasmid

#177809

Purpose

Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

Depositor

Sven Eyckerman

Article

Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

Type

Empty backbone

Use

Tags

12X MS2 stem loops

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-hTSC1-T2A-Bsd

Plasmid

#193199

Purpose

Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

TSC1 (TSC1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

Plasmid

#113684

Purpose

EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Cytomegalo Virus(CMV)

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235309

Purpose

ComMAND open-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EFS (human EF1a short, intronless)

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235310

Purpose

ComMAND closed-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EFS (human EF1a short, intronless)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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