We narrowed to 3,452 results for: ttl

1. pShuttle-EGFP-CLASP2 340-1362 9xS/A

   Plasmid

   #24373

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1362) 9xS/A (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen

   Plasmid

   #207123

   Purpose

   Lentiviral vector to express human METTL3

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   hMETTL3 (METTL3 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-C-Myc-DDK-IRES-Puro METTL3

   Plasmid

   #200724

   Purpose

   Lentiviral expression of Flag-Tagged human METTL3

   Depositor

   Claudio Alarcón

   Insert

   METTL3 (METTL3 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pShuttle-EGFP-CLASP2 340-1084 9xS/A

   Plasmid

   #24384

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (340-1084) 9xS/A (CLASP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable CLASP2 deletion mutant. M…

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

   Plasmid

   #182346

   Purpose

   PacI-flanked CAG-LSL expression cassette with a FNF for positive selection

   Depositor

   Mario Capecchi

   Insert

   tdTomato
   

   Use

   Mouse Targeting; Flp / frt

   Expression

   Mammalian

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

   Plasmid

   #207136

   Purpose

   DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9_CL

   Plasmid

   #166245

   Purpose

   regulated dCas9 generator

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   P112-sgRNA-term-P104-RBS1-dCas9-B15
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and Ec-TTL-P104

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAUX_OL

   Plasmid

   #166246

   Purpose

   use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

   Insert

   [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
   

   Expression

   Bacterial

   Promoter

   Ec-TTL-P112 and BBa_J23116

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH29_32 series plasmid

   Plasmid

   #120901

   Purpose

   Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

   Depositor

   Domitilla Del Vecchio

   Article

   Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

   Inserts

   Ec-TTL-P109
   sRNA-A targeting sequence
   RBS TIR=974 of GFPop1 gene
   GFPop1
   RBS TIR=6474 of ECF32mut gene
   ECF32mut
   T7 terminator
   resource competitor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R363

    Plasmid

    #138233

    Purpose

    Expresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(NGK).Ckl-Ter*.(TTL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. M3-M14-dCas9

    Plasmid

    #134779

    Purpose

    Expression of fusion protein M3-M14-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK1037

    Plasmid

    #8833

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease, catalytic domain
    

    Expression

    Bacterial

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK1035

    Plasmid

    #8832

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease
    

    Tags

    His and MBP (cleaved)

    Expression

    Bacterial

    Mutation

    synthetic gene with codons optimized for E. coli

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHPK1212

    Plasmid

    #8834

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    tobacco vein mottling virus protease, catalytic domain
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHel3

    Plasmid

    #102961

    Purpose

    E.coli/H.Pylori shuttle vector

    Depositor

    Rainer Haas

    Article

    Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

    Type

    Empty backbone

    Use

    Shuttle vector

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHel2

    Plasmid

    #102960

    Purpose

    E.coli/H.Pylori shuttle vector

    Depositor

    Rainer Haas

    Article

    Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

    Type

    Empty backbone

    Use

    Plasmid shuttle vector system for genetic complem…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBSWt1-Dest

    Plasmid

    #59764

    Purpose

    PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette

    Depositor

    Peter Koopman

    Article

    Zhao et al Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.

    Insert

    attL1-em7-kanR-attL2-pA
    

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMin1

    Plasmid

    #102959

    Purpose

    Mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusions

    Depositor

    Rainer Haas

    Article

    Kahrs et al Gene. 1995 Dec 29;167(1-2):53-7.

    Type

    Empty backbone

    Use

    Mini-transposon system suitable for sequencing, s…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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