We narrowed to 1,621 results for: sp1

1. LV-AR-R599A-N600A

   Plasmid

   #171221

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-R599A-N600A (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-R599A-N600A

   Promoter

   EF-1a

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LV-AR-AAFF

   Plasmid

   #171218

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-AAFF (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-K580A-V581A

   Promoter

   EF-1a

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

   Plasmid

   #136652

   Purpose

   Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2553

   Plasmid

   #142958

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   AR (AR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-hAR-R617M,K618M,K632M,K633M

   Plasmid

   #115778

   Purpose

   Expresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   Androgen Receptor (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R617M, K618M ,K632M, K633M, insertion of Q81 and …

   Promoter

   CMV

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-M4-AR-W741C-S650A

   Plasmid

   #171252

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys-Ser650Ala mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-M4-AR-W741C-S650D

   Plasmid

   #171253

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3xFlag-tagged human androgen receptor Trp741Cys-Ser650Asp mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)

   Promoter

   CMV

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

   Plasmid

   #225944

   Purpose

   Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Evan Reid, Richard Eva

   Inserts

   ATL1 (ATL1 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCA528 CHMP1B 143-199

   Plasmid

   #108255

   Purpose

   Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP1B residues 143-199 (CHMP1B Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCA528 CHMP4B 156-224

    Plasmid

    #104596

    Purpose

    Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B residues 156-224 (CHMP4B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Contains nonnative Gly-Cys for fluorescent labeli…

    Promoter

    T7

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pb-miRE-tre-3xFLAG-hnRNPU∆RGG

    Plasmid

    #196088

    Purpose

    Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAF-A (Hnrnpu Mouse)
    

    Use

    Tags

    N-terminal 3xFLAG

    Expression

    Bacterial and Mammalian

    Mutation

    removed last 154 amino acids on cterminal end

    Promoter

    TRE

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCG_SARS-CoV1 NSP7 C-V5-tag

    Plasmid

    #179963

    Purpose

    Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LAMTOR3 gRNA (BRDN0001147669)

    Plasmid

    #77136

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LAMTOR3 gRNA (BRDN0001145475)

    Plasmid

    #77134

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LAMTOR3 gRNA (BRDN0001145977)

    Plasmid

    #77135

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LAMTOR3 gRNA (BRDN0001147910)

    Plasmid

    #77137

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human LAMTOR3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    LAMTOR3 (LAMTOR3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pinducer 20 DN-KASHΔPPPL

    Plasmid

    #129280

    Purpose

    Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

    Depositor

    Daniel Conway

    Article

    Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

    Insert

    SYNE1 (SYNE1 Human)
    

    Use

    Lentiviral

    Tags

    mcherry

    Expression

    Mutation

    the last 4 amino acids (PPPL) of the KASH domain …

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-hAR-H874Y

    Plasmid

    #89087

    Purpose

    mammalian expression of human androgen receptor with prostate cancer somatic mutation H874Y from CWR22 xenograft

    Depositor

    Elizabeth Wilson

    Article

    Tan et al Mol Endocrinol. 1997 Apr;11(4):450-9. do

    Insert

    hAR-H874Y prostate cancer mutant (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-H874Y

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

19. AR-V7-pcw107

    Plasmid

    #64635

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AR (transcript variant 1, splice isoform) (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    splice isoform*

    Promoter

    PGK

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AR-V7-pcw107-V5

    Plasmid

    #64636

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    AR (transcript variant 1, splice isoform) (AR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    splice isoform *

    Promoter

    PGK

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only