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We narrowed to 6,480 results for: poly

Showing: 3951 - 4000 of 6480 results

  1. p5A1-Ti

    Plasmid
    #92018
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V, C151A
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92018-plasmid-map-sequence-id-190014

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  5. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92020-plasmid-map-sequence-id-190036

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  6. p5A3-T

    Plasmid
    #92019
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92019-plasmid-map-sequence-id-190012

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  9. pJB65

    Plasmid
    #226319
    Purpose
    E. coli protein expression for protein purification
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    6xHis- (VTG to AAA MR1-6)-strep
    Use
    Tags
    6xHis and Strep
    Expression
    Bacterial
    Mutation
    N-terminal domain deleted, C-terminal domain dele…
    Promoter
    T7
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    226319-plasmid-map-sequence-id-449981

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  14. p5A1-T

    Plasmid
    #92017
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    92017-plasmid-map-sequence-id-201852

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  15. p5C3-T

    Plasmid
    #92022
    Purpose
    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92022-plasmid-map-sequence-id-190017

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  25. pSR657

    Plasmid
    #65214
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-2 cap
    Use
    AAV
    Tags
    Expression
    Insect
    Mutation
    Promoter
    p10 and polyhedrin
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65214-plasmid-map-sequence-id-116341

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  27. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  30. pSR646

    Plasmid
    #65215
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-6 cap
    Use
    AAV
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P10 and polyhedrin
    Available since
    May 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65215-plasmid-map-sequence-id-112598

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  32. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119868-plasmid-map-sequence-id-240979

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  34. pCAGGS_NP_EBOV

    Plasmid
    #103049
    Purpose
    Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter
    Depositor
    Elke Mühlberger
    Article
    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do
    Insert
    NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    silent mutation (T to C) at position 928 of NP ge…
    Promoter
    CAG
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    103049-plasmid-map-sequence-id-197238

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  37. pAluYa5-neo-TET

    Plasmid
    #51283
    Purpose
    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    AluYa5
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    upstream pol III enhancer sequence of the 7SL gen…
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51283-plasmid-map-sequence-id-78362

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  38. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid
    #221075
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Article
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Insert
    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221075-plasmid-map-sequence-id-438457

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  47. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Article
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Tags
    Expression
    Insect
    Mutation
    Promoter
    P10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    65216-plasmid-map-sequence-id-115626

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Showing: 3951 - 4000 of 6480 results