We narrowed to 11,716 results for: VARS
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Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK671.306
Plasmid#192641PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyPromoterBBa_J23119Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZH519
Plasmid#102672PurposeTetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH522
Plasmid#102675PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSc polyA + Shal1
Plasmid#16196DepositorInsertDrosophila melanogaster Shaker cognate l (Shal Fly)
UseXenopus oocyte expressionMutationN/AAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
GTH-r
Plasmid#81106PurposeGene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST-KNarK2
Plasmid#44565Purposefor ectopic hypoxia inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω1
Plasmid#101710PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-TagRFP675-N1
Plasmid#44276DepositorInsertTagRFP675
Tagschicken paxillinExpressionMammalianMutationM41Q/F80W/S143N/L147M/S158N/D159Y/N173SPromoterCMVAvailable SinceOct. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-CAHS1
Plasmid#90030PurposeTo express GFP-tagged CAHS1 in mammalian cells transientlyDepositorInsertCAHS1
TagsAcGFP1ExpressionMammalianPromoterCMVAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQP-2460
Plasmid#138973PurposeLight-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
Available SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4-2T
Plasmid#28250DepositorInsertsingle chain Cse4-Scm3-H4 with 2 Thrombin site
TagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3. An…Available SinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
Tags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBIP1::eYFP
Plasmid#135231PurposeUPR signaling reporter (upregulates eYFP expression under UPR conditions)DepositorInsertpBiP1
TagseYFPExpressionPlantPromoterBiP1Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-VSVG-16
Plasmid#55517PurposeLocalization: Golgi/ER Transport, Excitation: 462, Emission: 492DepositorInsertVSVG
TagsmTFP1ExpressionMammalianMutationdeltaK VSVG ts045PromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)
Plasmid#159886PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-1(no_atg)
ExpressionWormMutationNot applicableAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)
Plasmid#159887PurposeThree-fragment Gateway compatible promoter with PATCs for germline expression from arraysDepositorInsert[4-1] - Promoter - Psmu-2 (no_start)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
h-alpha-ENaC-2
Plasmid#83431Purposefull length human alpha ENaC-2 with flag tagDepositorAvailable SinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1a
Plasmid#98705Purposemammalian expression of Glt1aDepositorInsertGlt1a (Slc1a2 Rat)
ExpressionMammalianMutationI288V (please see depositor comments below)PromoterCMVAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A) C716A
Plasmid#24605DepositorAvailable SinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVPSB_empty
Plasmid#192170PurposepVPSB backbone expression vectorDepositorTypeEmpty backboneExpressionMammalianMutationNAAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZH512
Plasmid#102666PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-flag-UTR
Plasmid#183234PurposeMammalian expression empty backboneDepositorTypeEmpty backboneTagsFLAG tagExpressionMammalianPromoterCMVAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB035a
Plasmid#79190PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
ExpressionBacterialPromoterPlac62; PtetAvailable SinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-153/47-VR-NG
Plasmid#50704PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagPromoterCMVAvailable SinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pML104-kanR1136
Plasmid#162040PurposeExpresses Cas9 and contains kanR1136 guide which targets the kanR geneDepositorInsertkanR1136 guide
UseCRISPRExpressionYeastAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS10: pTns(AvCAST)_ΔTnsD
Plasmid#168143PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.DepositorInsertAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 sgACO1_1
Plasmid#169885PurposeDisrupt ACO1DepositorInsertsgRNA targeting ACO1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR V2 sgACO1_2
Plasmid#169892PurposeDisrput ACO1DepositorInsertsgRNA targeting ACO1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1b
Plasmid#98706Purposemammalian expression of Glt1bDepositorInsertGlt1b (Slc1a2 Rat)
ExpressionMammalianMutationI348V and N365Y (please see depositor comments se…PromoterCMVAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Lysosomes-20
Plasmid#55495PurposeLocalization: Lysosome Membrane, Excitation: 462, Emission: 492DepositorInsertLAMP1
TagsmTFP1ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HisSumoOphP
Plasmid#189672PurposeHisSUMO tagged OphP to express OphP in pichia pastorias.DepositorInsertHisSumoOphP
TagsHisSumo taggedExpressionYeastAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
GTH-g
Plasmid#81104PurposeGene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1360 - Intron GG3 - smu-1
Plasmid#159806PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1358 - Intron GG1 - smu-1
Plasmid#159804PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1386
Plasmid#111445PurposeSolo vector pV1382 + sgScADE2DepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRExpressionYeastAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH511
Plasmid#102665PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only