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Showing: 1601 - 1620 of 23409 results

  7. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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  9. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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  14. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65769-plasmid-map-sequence-id-112659

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  17. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Andreas Weber
    Article
    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86556-plasmid-map-sequence-id-176429

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  19. pCB-CBEv2

    Plasmid
    #221139
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221139-plasmid-map-sequence-id-437031

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  20. pNHEJ-Cas12a-recX

    Plasmid
    #158714
    Purpose
    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    recX
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pmyc
    Available since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    158714-plasmid-map-sequence-id-321929

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Showing: 1601 - 1620 of 23409 results