We narrowed to 59,686 results for: plasmid
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Plasmid#169739PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ACE2-his expression plasmid
Plasmid#158089PurposeExpresses ACE2 in mammalian cellsDepositorAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ER-mit RspA-splitFAST (lentiviral plasmid)
Plasmid#233604PurposeExpression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragmentsDepositorInsertRspA-splitFAST
UseLentiviralPromoterCMV/SV40Available sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
CBS-3621
Plasmid#255702PurposeNon-target expressionDepositorInsertSelf-non target plasmid encoding CAO1 guide target under PJ23119 promoter GAAAG PFS
ExpressionBacterialMutationN/APromoterJ23119Available sinceAug. 26, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMAT_M4_YVTM_YQNQ
Plasmid#197084PurposeThis plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFPDepositorInsertThis plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs.
ExpressionMammalianAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Shu
Plasmid#186655PurposeDonor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
Tags3xFlag-3xHAExpressionInsectAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
ExpressionMammalianAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACP-GPI Control Plasmid
Plasmid#101131PurposeExpression of ACP-Tagged GPI anchor in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPI
TagsACP-Tag (ACP wt)ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
ppk301-T2A-QF2 HDR plasmid
Plasmid#130667PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
UseDonor templateAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Noodles Plasmid
Plasmid#212613PurposeAn all-in-one system for on-target efficiency analysis of CRISPR guide RNAsDepositorTypeEmpty backboneUseCRISPR and LuciferaseExpressionMammalianAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + filler DNA reporter plasmid
Plasmid#156454PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsNeoR/KanR
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACP-ADRβ2 Control Plasmid
Plasmid#101128PurposeExpression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeta-2-Adrenergic Receptor (ADRB2 Human)
TagsACP-tagExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)
Plasmid#223137PurposepU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)DepositorInsertetevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
UseCRISPRExpressionMammalianPromoterU6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
BRD4 CRISPRi plasmid
Plasmid#154890PurposeHuman BRD4 CRISPRi gRNADepositorInsertBRD4-targeting gRNA
UseCRISPR and LentiviralAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only