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pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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R777-E283 Hs.ROCK1

Plasmid

#70567

Purpose

Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ROCK1 (ROCK1 Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

Plasmid

#122147

Purpose

Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

NLS-iLID-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti NbALFA-mNeonGreen hygro

Plasmid

#193974

Purpose

lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance

Depositor

Raphael Gaudin

Article

Bare et al bioRxiv 2025.10.07.680694

Insert

NbALFA-mNeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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tdTomato-H2B-6

Plasmid

#58101

Purpose

Localization: Nucleus/Histones, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

H2B (H2BC11 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

Plasmid

#20942

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

EF1alpha

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET28b-ULP1-405-621

Plasmid

#229224

Purpose

For expression of ULP1 protein

Depositor

Andy LiWang

Insert

ULP1 (ULP1 Budding Yeast)

Expression

Bacterial

Mutation

405-621 (truncated)

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCas9

Plasmid

#162606

Purpose

Pre-express Cas9 in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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