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pMDC123SB-AtMIR390a-B/c

Plasmid

#51775

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Article

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pVITRO1-M80-F2-IgG1/κ

Plasmid

#50383

Purpose

Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

human Gamma 1 heavy chain expression cassette with unknown specificity
human Kappa light chain expression cassette with unknown specificity

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

Jan. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRK GFP Paxillin

Plasmid

#50529

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Insert

Paxillin (PXN Human)

Tags

GFP21

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pKIKOlacZCm

Plasmid

#46764

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+/ER-2mutAEQ

Plasmid

#45541

Depositor

Javier Alvarez-Martin

Article

de la Fuente et al Biochem J. 2012 Aug 1;445(3):371-6. doi:

Insert

ER-2mutAEQ

Tags

ER targeted

Expression

Mammalian

Mutation

Asp119Ala and Asn28Leu

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA6A-EGFR ECD (1-644)

Plasmid

#42666

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR ECD (1-644) (EGFR Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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BRD4

Plasmid

#38942

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BRD4 (BRD4 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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drebrin-YFP

Plasmid

#40359

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Use

Chick expression

Tags

eYFP

Promoter

chick B-actin promoter with CMV enhancer

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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