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Showing: 2431 - 2460 of 20871 results

  3. pRRL-VinculinTS-S1033A

    Plasmid
    #211835
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinTS-S1033A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211835-plasmid-map-sequence-id-417709

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  4. pRRL-VinculinTS-S1033D

    Plasmid
    #211893
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinTS-S1033D (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211893-plasmid-map-sequence-id-419257

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  5. pRRL-VinculinCS-S1033A

    Plasmid
    #211894
    Purpose
    Vinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinCS-S1033A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211894-plasmid-map-sequence-id-417711

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  6. pRRL-VinculinCS-S1033D

    Plasmid
    #211895
    Purpose
    Vinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinCS-S1033D (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211895-plasmid-map-sequence-id-419255

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  7. pGEX-2T-CBP(1-287)

    Plasmid
    #182839
    Purpose
    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    CBP1-287
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182839-plasmid-map-sequence-id-360063

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  8. pPLN-hFluc-DM-HA-GFP11-KDEL

    Plasmid
    #177723
    Purpose
    DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFlucDM
    Use
    Tags
    GFP11, HA, KDEL, and Prolactin Signal Sequence
    Expression
    Bacterial and Mammalian
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177723-plasmid-map-sequence-id-352618

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  9. p406 – TDH3-ER-FlucDM-GFP- Ura

    Plasmid
    #177732
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    HA, HDEL, KAR2 Signal Sequence, and eGFP
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177732-plasmid-map-sequence-id-352622

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  10. p5E-161K enhancer-NR2F2-MUT

    Plasmid
    #138371
    Purpose
    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    161k human enhancer with conserved NR2F2 binding sites mutated
    Use
    Gateway compatible
    Tags
    Expression
    Mutation
    NR2F2 sites mutated
    Promoter
    none
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138371-plasmid-map-sequence-id-272006

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  11. p5E-161K enhancer-ETS-CORE-MUT

    Plasmid
    #138372
    Purpose
    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    human 161K human enhancer with ETS bind sites mutated
    Use
    Gateway compatible
    Tags
    Expression
    Mutation
    ETS factor binding sites mutated
    Promoter
    NONE
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138372-plasmid-map-sequence-id-272008

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  12. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

    Plasmid
    #108378
    Purpose
    FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108378-plasmid-map-sequence-id-209430

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  13. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

    Plasmid
    #108377
    Purpose
    FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108377-plasmid-map-sequence-id-209424

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  21. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209416-plasmid-map-sequence-id-416613

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  24. pTXB1-pA-M.EcoGII

    Plasmid
    #192873
    Purpose
    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578
    Depositor
    Chong Tang
    Article
    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:
    Insert
    pA-M.EcoGII
    Use
    Tags
    3X Flag Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    192873-plasmid-map-sequence-id-380474

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  25. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

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Showing: 2431 - 2460 of 20871 results