We narrowed to 6,931 results for: crispr cas9 plasmids

1. pXEE401B

   Plasmid

   #91716

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK02

   Plasmid

   #105133

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM126

   Plasmid

   #105134

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAT11

   Plasmid

   #204290

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT75

   Plasmid

   #204306

   Purpose

   Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT62

   Plasmid

   #204301

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJAT43

    Plasmid

    #204299

    Purpose

    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT56

    Plasmid

    #204300

    Purpose

    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-ECFP

    Plasmid

    #112220

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    ECFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSc1

    Plasmid

    #80436

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SCP1-dSa-VPR mini.

    Plasmid

    #99685

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EFS-dSa-VPR mini.

    Plasmid

    #99686

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. BPK2797

    Plasmid

    #72251

    Purpose

    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335Q, and T1337R

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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