We narrowed to 8,907 results for: Pol

1. pQ54-VC

   Plasmid

   #175060

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ54) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ54 (HTT Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQ25-VC

   Plasmid

   #175062

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ25) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ25 (HTT Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQ39-VC

   Plasmid

   #175061

   Purpose

   expresses mVenus and mCherry tagged to a mutant of the huntingtin exon1 (polyQ39) in mammalian cells

   Depositor

   Arnold Boersma

   Article

   Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

   Insert

   polyQ39 (HTT Human)
   

   Use

   Tags

   mVenus + mCherry

   Expression

   Mammalian

   Mutation

   mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

   Promoter

   CMV Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-SopB

   Plasmid

   #183657

   Purpose

   N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVITRO1-102.1F10-IgG4/λ

   Plasmid

   #50369

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG4/λ antibody isotype (102.1F10-IgG4/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 4 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVITRO1-102.1F10-IgA2/λ

   Plasmid

   #50371

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVITRO1-102.1F10-IgG2/λ

   Plasmid

   #50367

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG2/λ antibody isotype (102.1F10-IgG2/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 2 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVITRO1-102.1F10-IgA1/λ

   Plasmid

   #50370

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgA1/λ antibody isotype (102.1F10-IgA1/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Alpha 1 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVITRO1-102.1F10-IgG3/λ

   Plasmid

   #50368

   Purpose

   Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
   grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eDrHER2

    Plasmid

    #183995

    Purpose

    Optically controlled eDrHER2 expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrHER2 (ERBB2 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. eDrFGFR1

    Plasmid

    #183994

    Purpose

    Optically controlled eDrFGFR1 expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrFGFR1 (FGFR1 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EGFP-IpgD(C439S)

    Plasmid

    #183667

    Purpose

    N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C439S

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His6-EGFP-PH-PLCD1

    Plasmid

    #183675

    Purpose

    PtdIns(4,5)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLCD1 (PLCD1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 11-140

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDTFendoNA2

    Plasmid

    #174731

    Purpose

    Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. G31, Cdc20-AID tagging

    Plasmid

    #130269

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)
    

    Use

    Tags

    AID(71-114)

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SopB-EGFP

    Plasmid

    #183655

    Purpose

    C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pESC-LEU-SopB

    Plasmid

    #183670

    Purpose

    Inducible Salmonella Typhimurium SopB for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pESC-Leu-SopB(C460S)

    Plasmid

    #183671

    Purpose

    Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C460S

    Promoter

    GAL1

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-SRGAP2A-FBAR-mRFP

    Plasmid

    #168502

    Purpose

    Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

    Depositor

    Franck Polleux

    Article

    Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

    Insert

    SRGAP2A-FBAR (SRGAP2 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium, Budding Yeast)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIDTSmart-CMV-PLRS1

    Plasmid

    #217365

    Purpose

    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    leucyl-tRNA synthetase polyspecific mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M40I, T252A, Y499I, Y527A, H529G

    Promoter

    CMV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG_SRGAP2A_SH3(W765A)_GFP

    Plasmid

    #222616

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG_SRGAP2A_SH3(W765A)_HA

    Plasmid

    #222614

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG SRGAP2A_EVH1(P339L F342C)

    Plasmid

    #222613

    Purpose

    Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutations P339L and F342C

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pet28b_His_RAD23A-UBA1

    Plasmid

    #201549

    Purpose

    Expresses UBA1 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 160-205 of DNA polymerase i…

    Promoter

    T7

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pet28b-His_RAD23A-UBA2

    Plasmid

    #201550

    Purpose

    Expresses UBA2 of human RAD23A in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 311-363 of DNA polymerase i…

    Promoter

    T7

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-SopB(K525A)

    Plasmid

    #183659

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K525A

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-SopB(K528A)

    Plasmid

    #183660

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K528A

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-SopB(33-554)

    Plasmid

    #183661

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 33-554

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-SopB(33-520)

    Plasmid

    #183662

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 33-520

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge