We narrowed to 4,238 results for: pes;

1. pCDH-EF1a-mCherry-EGFP-LC3B

   Plasmid

   #170446

   Purpose

   Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   mCherry-GFP-LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1a-hDNAJC6-T2A-copGFP

   Plasmid

   #170443

   Purpose

   Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   human DNAJC6 (DNAJC6 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSC-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90214

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHDR-FOXL2-T2A-tdTomato-PuroTK

   Plasmid

   #192892

   Purpose

   HDR donor for inserting FOXL2-T2A-tdTomato reporter

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOXL2-tdTomato (FOXL2 Human)
   

   Use

   CRISPR

   Mutation

   T2A-tdTomato fluorescent reporter

   Promoter

   PGK

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSC-ISL1-T2A-LHX3

   Plasmid

   #90215

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   ISL1-T2A-LHX3 (ISL1, LHX3 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-tetO-NB

   Plasmid

   #197092

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-Blast-DNFGFR1-HA

   Plasmid

   #130887

   Purpose

   Expresses dominant negative mutant mouse FGFR1

   Depositor

   Gian-Paolo Dotto

   Article

   Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

   Insert

   Fgfr1 (Fgfr1 Mouse)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Dominant Negative receptor lacking cytosolic cata…

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-FRT-Rev-TdTomato

   Plasmid

   #191203

   Purpose

   FlpO dependent TdTomato expression

   Depositor

   Janelia Viral Tools

   Article

   Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

   Insert

   TdTomato
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-FRT-Rev-3xGFP

   Plasmid

   #191204

   Purpose

   FlpO dependent GFP expression

   Depositor

   Janelia Viral Tools

   Article

   Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

   Insert

   3X GFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-RPS14(WT)

    Plasmid

    #122297

    Purpose

    expresses RPS14 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-Hf-RfxCas13d-HA

    Plasmid

    #234479

    Purpose

    Plasmid to carry out IVT of High-fidelity RfxCas13d (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    HA

    Mutation

    Changed four alanine to valine in positions 134, …

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Myc-CyclinD1(WT)

    Plasmid

    #122300

    Purpose

    expresses Cyclin D1 protein with a Myc tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    CCND1 (CCND1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATX3-77Q

    Plasmid

    #184249

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Codon optimization for protein expression in BL2…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid

    #111508

    Purpose

    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    VPR-dSpCas9[StaPL(TI)]
    

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, and S122…

    Promoter

    PGK

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112214

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Mutation

    D10A,H840A,N863A

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

    Plasmid

    #112210

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNA methyltransferase 3 alpha (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Mutation

    D10A,H840A,N863A

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112209

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3L, DNMT3A Human)
    

    Use

    CRISPR

    Mutation

    D10A,H840A,N863A,C710S

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN-L1-K2-L2

    Plasmid

    #80684

    Purpose

    Gateway Entry vector containing a temperature-sensitive mouse DHFR

    Depositor

    Nico Dissmeyer

    Article

    Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

    Inserts

    DHFR (Dhfr Mouse)
    DHFR (Dhfr Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    mouse DHFR with N-terminal Ubiquitin

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MIGR1-Cdc42-1

    Plasmid

    #179517

    Purpose

    Retroviral expression of Cdc42 splice variant 1

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 1 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112215

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Mutation

    D10A,H840A,N863A,C710S

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBABE-RPS14(WT)-Myc

    Plasmid

    #122235

    Purpose

    expresses RPS14 protein with a Myc tag in mammalian cells (puro resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    vector's LTR

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MIGR1-Cdc42-2

    Plasmid

    #179518

    Purpose

    Retroviral expression of Cdc42 splice variant 2

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 2 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ATX3-77Q-R388G

    Plasmid

    #184251

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Lenti-LucAL

    Plasmid

    #174049

    Purpose

    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucAL
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…

    Promoter

    Human Ubiquitin C

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT3TS-RfxCas13d-2C-NLS

    Plasmid

    #239816

    Purpose

    Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    Nucleoplasmin long NLS and SV40 NLS

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28b-Hf-RfxCas13d-His

    Plasmid

    #234480

    Purpose

    Plasmid for bacterial expression and purification of High-fidelity RfxCas13d protein (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed four alanine to valine in positions 134, …

    Available Since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

    Plasmid

    #231233

    Purpose

    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    MYC (MYC Human)
    

    Expression

    Mammalian

    Mutation

    TAD deleted (delta1-143); numbering based on sequ…

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TR01F/hFGFR3-G380R-V5

    Plasmid

    #214908

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR3-G380R variant that is associated with Achondroplasia

    Depositor

    Pavel Krejčí

    Article

    Kimura et al Sci Transl Med. 2021 May 5;13(592):eaba4

    Insert

    FGFR3 (FGFR3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    G380R substitution

    Promoter

    truncated CMV promoter

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ATX3 13Q- I77K Q78K W87K

    Plasmid

    #185908

    Purpose

    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Point mutations in Atx3 gene I77K Q78K and W87K

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Venus-BimEL-4E-pEGFP-C1

    Plasmid

    #166742

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    BimEL-4E (Bcl2l11 Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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