We narrowed to 8,600 results for: Ott

1. pBABE FLAG HA p100

   Plasmid

   #236468

   Purpose

   stable expression of p100 using virus infection

   Depositor

   Michele Pagano

   Article

   Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

   Insert

   p100 (NFKB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

   Plasmid

   #238372

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cmlc2 (myl7)

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTol1-Spe-KpnI-ubb-nmKate

   Plasmid

   #238373

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-HA

   Plasmid

   #235678

   Purpose

   Plasmid control for pcDNA3.1-HA-RAD52-WT and pcDNA3.1-HA-RAD52-∆C

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. iNicSnFR12

   Plasmid

   #227142

   Purpose

   Genetically encoded fluorescent biosensor for nicotine detection

   Depositor

   Henry Lester

   Article

   Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Insert

   iNicSnFR12
   

   Use

   Tags

   6xHis-HA and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pToxEXPr6

   Plasmid

   #218290

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJQR3

   Plasmid

   #218289

   Purpose

   Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pITEdv1

   Plasmid

   #218283

   Purpose

   Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pITEu2

   Plasmid

   #218282

   Purpose

   Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJE13HD7

    Plasmid

    #218281

    Purpose

    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-hXRCC1pd-HIS

    Plasmid

    #206036

    Purpose

    Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    T7

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Silene_conica _GlnRS_MitoTP_pK7FWG2

    Plasmid

    #202654

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from Silene conica cytosolic GlnRS predicted to be re-targeted to mitochondria
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Silene_conica _LysRS_MitoTP_pK7FWG2

    Plasmid

    #202655

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from Silene conica cytosolic LysRS predicted to be re-targeted to mitochondria
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. Agrostemma_githago _LysRS_MitoTP_pK7FWG2

    Plasmid

    #202656

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from Agrostemma githago cytosolic LysRS predicted to be re-targeted to mitochondria
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Silene_conica _TyrRS_MitoTP_pK7FWG2

    Plasmid

    #202657

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from Silene conica cytosolic TyrRS predicted to be re-targeted to mitochondria
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Silene_vulgaris _TyrRS_MitoTP_pK7FWG2

    Plasmid

    #202658

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from Silene vulgaris cytosolic TyrRS predicted to be re-targeted to mitochondria
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Silene_conica _PheRS_MitoTP_pK7FWG2

    Plasmid

    #202659

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to mito
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. Silene_conica _PheRS_PlastidTP_pK7FWG2

    Plasmid

    #202660

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from organellar Silene conica PheRS predicted to be targeted to plastid
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Arabidopsis_thaliana _PheRS_DualTP_pK7FWG2

    Plasmid

    #202661

    Purpose

    Determine where transit peptide targets GFP in N. benthamiana

    Depositor

    Daniel Sloan

    Article

    Warren et al Mol Biol Evol. 2023 Jul 5;40(7):msad163.

    Insert

    N-terminal transit peptide from organellar Arabidopsis thaliana PheRS known to be dual targeted to plastid and mitochondria
    

    Use

    Tags

    Green Florescent Protien

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBAD-P19-EGFP

    Plasmid

    #196609

    Purpose

    siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + EGFP inverted repeat sequence
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBAD-P19-NP

    Plasmid

    #196608

    Purpose

    siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    p19 + Influenza virus NP inverted repeat
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. psiCHECK-NP

    Plasmid

    #196607

    Purpose

    Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    nucleocapsid protein (NP) gene, partial
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tetO-Fzd1-IRES-tdTomato (TET006)

    Plasmid

    #160510

    Purpose

    This plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

    Depositor

    C. Ron Yu

    Article

    Wu et al bioRxiv 2022

    Insert

    Fzd1 (Fzd1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pILGFP4D41

    Plasmid

    #185841

    Purpose

    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pILGFP10CF72 (B11)

    Plasmid

    #185859

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pILGFP8EFA

    Plasmid

    #185854

    Purpose

    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    DHFR P66L

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-ZmIAA29 (yeast codon optimized)

    Plasmid

    #188441

    Purpose

    Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon optimization

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Repressor IAA29 (yeast codon optimized)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 1056H

    Plasmid

    #183135

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1056J

    Plasmid

    #183136

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 1056K

    Plasmid

    #183137

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSF-G1pCNPRS

    Plasmid

    #174719

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPLN-hFluc-WT-eGFP-KDEL

    Plasmid

    #177727

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    KDEL, Prolactin Signal Sequence, and eGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p406 – TDH3-MTS-GFP11-Ura

    Plasmid

    #177733

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-GFP11
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPLN-hFluc-WT-KDEL

    Plasmid

    #177715

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    KDEL and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. actc1b-mKate2-P2A-APEX2-VHH[GFP]

    Plasmid

    #126749

    Purpose

    Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    VHH[EGFP]
    

    Use

    Zebrafish muscle expression clone

    Tags

    mKate2-P2A-APEX2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p1.2-Zeo-hVKORC1

    Plasmid

    #162776

    Purpose

    Human vitamin K oxidoreductase expression

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

    Insert

    VKORC1 vitamin K epoxide reductase complex subunit 1 (VKORC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CMV-10xQb oligo-library based

    Plasmid

    #158201

    Purpose

    Cassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of QB based on oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBbdCas9S(-10AC1)_Psyn-sgRNArodA

    Plasmid

    #149659

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC1), Psyn

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBbdCas9S(-10AC2)_Psyn-sgRNArodA

    Plasmid

    #149660

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC2), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

    Plasmid

    #149591

    Purpose

    all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBbdCas9S(-10TC)_Psyn-sgRNArodA

    Plasmid

    #149657

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBbdCas9S(-10TC)_Psyn-sgRNAflaB

    Plasmid

    #149589

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET_4E2H.210

    Plasmid

    #155212

    Purpose

    Expresses 4E2H.210 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. D38_Ld_341960

    Plasmid

    #135852

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    thioredoxin, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. D32_Ld_354780

    Plasmid

    #135848

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    Hsp70 protein, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. D63_Ld_100680

    Plasmid

    #135876

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    flagellar glycoprotein-like protein
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge